E line spectrometer

The solvents were subjected to previous purification and drying processes by standard methods. The progress of the reactions and purity of the synthesized compounds was evaluated by thin-layer chromatography (TLC) using silica gel 60 plates with UV254 fluorescent indicator, an ultraviolet lamp and a suitable solvent system.
Melting points were measured with a Stuart™ SMP3 melting point apparatus (Sigma-Aldrich). Infrared spectra were recorded on KBr pellets with a Shimadzu FTIR 8400 spectrophotometer (400-4000 cm^-1). Nuclear Magnetic Resonance spectra were taken using two spectrometers, one of Jeol Eclipse brand and another Bruker Avance II brand, of 270 MHz and 300 MHz for ¹H NMR, respectively. Both devices have the power of 67.9 MHz for ¹³C NMR.
Electron Paramagnetic Resonance (EPR) spectra were taken at 24 °C on a reconstructed and automated X-band Varian E-Line spectrometer. A rectangular cavity resonating in the TE-102 mode was used, with modulation of 100 kHz, using a microwave power of 5 mW. ESI-CID-MS² spectra were recorded using a TSQ Quantum triple-quadrupole mass spectrometer (Thermo Scientific brand) coupled to a liquid chromatography (CL) system.

CQDP, calf thymus DNA (CT-DNA), buffers and solvents were used as received from Sigma-Aldrich Co. All other commercial reagents were also used without further purification. The NMR spectra of the compounds were recorded in CH₂Cl₂-d₂ and DMSO-d₆ solution in Bruker AVANCE 300 MHz or 500 MHz spectrometers. IR spectra were recorded with a Perkin Elmer Series 100 spectrometer in the 4000–400 cm⁻¹ region, ultraviolet-visible (UV-Vis) spectra on a HP 8453 diode array spectrometer and fluorescence spectra on a Perkin Elmer LS45 fluorescence spectrometer. C, H and N analyses were performed using a Carlo Erba Model EA1108 elemental analyzer. ESI (+) MS spectra were obtained by direct infusion in a QTOF Bruker Impact II Mass Spectrometer in positive ion mode, utilizing CH₃CN (LC/MS grade from Honeywell; B&J Brand) as the solvent. Electron Paramagnetic Resonance (EPR) spectra were performed on a Varian E-Line spectrometer. In the β-hematin formation inhibition assay plates were centrifuged using a Thermo Scientific IEC CL 30 centrifuge and measured in a Tecan Sunrise Absorbance Reader at 405 nm.