Rat il 1β elisa kit

The expression of TNF-α, IL-1β, and IL-6 in the spinal cord was examined using the LEGEND MAX™ Rat TNF-α ELISA Kit (Biolegend, China), Rat IL-1β ELISA Kit (Dakewe, China), and LEGEND MAX™ Rat IL-6 ELISA Kit (Biolegend, China).

The culture mediums were collected and centrifuged to remove floating cells. The supernatant was used for ELISA assay. The expression of TNF-α, IL-1β, and IL-10 was measured using an ELISA kit in accordance with the protocol provided by the manufacturer. Briefly, different samples and the detection antibody were added to each well. After incubated for 30 min, the plates were washed, and aenzyme and chromogenic substrate were added. Then stop solution was added to terminate the reaction. Finally, the absorbance value at 450 nm was measured with a microplate reader to determine the concentration of each well. ELISA kits used were Rat cAMP ELISA kit (ab133039, Abcam), Rat TNF-α ELISA kit (12-3720, Dakewe, China), Rat IL-1β ELISA kit (12-3012, Dakewe, China), and Rat IL-10 ELISA kit(12-3100, Dakewe, China). Experiments were repeated at more than three times.