Cayman 11 kt eia kit

Manufactured by Cayman Chemical

Sourced in United States

The Cayman 11-KT EIA kit is a laboratory tool used to quantify the levels of 11-ketotestosterone, a steroid hormone, in various biological samples. It is designed to provide accurate and reliable measurements through an enzyme immunoassay (EIA) technique.

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Lab products found in correlation

Platereader af2200, by Eppendorf (1 mentions)

Close spelling variants of this product

Cayman-EIA kit Cayman kits EIA kit

2 protocols using cayman 11 kt eia kit

Quantification of Steroid Hormones

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Plasma levels of testosterone (T), 11-ketotestosterone (11-KT), and 17β-estradiol (E2) were quantified by ELISA, and each standard and plasma sample was run in duplicate.
Concentration of T (KAPD1559) and E2 (KAP0621) were analyzed by ELISA using commercially available kits (DIAsource, Ottignies-Louvain-la-Neuve, Belgium) according to the manufacturer’s instructions. The androgen 11-KT was assayed with the Cayman 11-KT EIA kit (582751, Cayman Chemical, Ann Arbour, MI USA). The intra-assay coefficients of variation (calculated from the duplicate sample) were less than 7% in all tests and inter-assay coefficients of variation were less than 6.5%. Absorbance was read with a microplate plate reader (PlateReader AF2200, Eppendorf, Hamburg, Germany).

Knowles J., Vysloužil J., Policar T., Milla S., Holická M, & Podhorec P. (2022). Spawning Performance and Sex Steroid Levels in Female Pikeperch Sander lucioperca Treated with Poly(lactic-co-glycolic acid) Microparticles. Animals : an Open Access Journal from MDPI, 12(2), 208.

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Hormonal Quantification Protocol

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Testosterone (T, ng/mL) was assayed on 25 μL of plasma using the DIAsource Testosterone ELISA kit (DIAsource, KAPD1559). When necessary, a 1/2 dilution of the samples was performed. Sensitivity was 0.083 ng/mL, CV intra-assays were 1.5, 7.9 and 9.5%, and CV inter-assays were 7.6, 13.9 and 8.7% for 0.2, 2 and 16 ng/mL, respectively.
The androgen 11-keto testosterone (11-KT, ng/mL) was assayed on 50 μL of plasma diluted 1/50 in ELISA buffer using the Cayman 11-KT EIA kit (Cayman, 582751). Sensitivity was 1.3 pg/mL, CV intra-assays were 2.6, 1.9 and 8.4%, and CV inter-assays were 9.4, 9.6 and 12.1% for 0.78, 6.25 and 100 pg/mL, respectively.
17β-estradiol (E2, ng/mL) was assayed on 50 μL of plasma using the DIAsource E2-ELISA kit (DIAsource, KAP0621). When necessary, a 1/20 to 1/70 dilution of the samples was performed. Sensitivity was 5 pg/mL, CV intra-assays were 3, 2.3 and 2.6%, and CV inter-assays were 4.7, 4.1 and 2.4% for 37, and 880 pg/ mL, respectively.

Ben Ammar I., Milla S., Missaoui H., Ledoré Y., Teletchea F, & Fontaine P. (2018). Does constant photoperiod inhibit the onset of the reproductive cycle in northern pike (Esox lucius) males?. Fish physiology and biochemistry, 44(1).

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