β3 tubulin

Manufactured by Thermo Fisher Scientific

Sourced in United States

β3-tubulin is a protein that is a component of the cytoskeleton in eukaryotic cells. It is a member of the tubulin family of proteins and is involved in the formation of microtubules, which are crucial for various cellular processes such as cell division, intracellular transport, and cell shape maintenance.

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Lab products found in correlation

Dmi6000b, by Leica (1 mentions) Dfc360 fx, by Leica (1 mentions) Dyelight 488, by Thermo Fisher Scientific (1 mentions) Gapdh, by Merck Group (1 mentions) Pabpc1, by Abcam (1 mentions) Psd95, by Cell Signaling Technology (1 mentions) V5 tag, by Santa Cruz Biotechnology (1 mentions) β actin, by BioLegend (1 mentions) Hnrnpa1, by Cell Signaling Technology (1 mentions)

2 protocols using β3 tubulin

Immunostaining of Human Dopaminergic Neurons

Cited 1 time

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Differentiated human dopaminergic neurons were fixed by using 4% paraformaldehyde for 15 min and permeabilised by Triton X-100 at 0.04% as described before^{28 (link)}. Primary antibodies against human aSYN (Santa Cruz C20; Santa Cruz Biotechnology, Dallas, USA), β3-tubulin (Invitrogen, Karlsruhe, Germany) at a concentration of 1:200 were incubated overnight at 4 °C, followed by secondary anti-rabbit, anti-mouse antibodies coupled to fluorophore Dyelight 488, and Dyelight 649 (Invitrogen) at a concentration of 1:500. Images were obtained by using a CCD camera (DFC 360, FX, Leica, Wetzlar, Germany) connected to an epifluorescent microscope (DMI 6000 B, Leica). For co-localisation studies, cells were co-stained with MitoTracker Deep Red (200 ng/ml) for mitochondria and DAPI (4′,6-diamidino-2-phenylindole, 1 µg/ml) for the nucleus. Quantification and analysis of neuronal network was performed by using the Image J software.

Ganjam G.K., Bolte K., Matschke L.A., Neitemeier S., Dolga A.M., Höllerhage M., Höglinger G.U., Adamczyk A., Decher N., Oertel W.H, & Culmsee C. (2019). Mitochondrial damage by α-synuclein causes cell death in human dopaminergic neurons. Cell Death & Disease, 10(11), 865.

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Antibody Acquisition and Generation

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Antibodies were purchased from Abcam (PABPC1, hnRNPA1, PSD-95, RPS6, and synaptophysin), Cell Signaling Technologies (PABPC1, V5, eIF4G, and β-Actin), BioLegend (β3tubulin), Invitrogen (V5 tag), Santa Cruz Biotechnology (GAPDH), and Sigma-Aldrich (PAIP2 and FLAG). A peptide encompassing the C-terminal end of mouse neuPABP (ERGAWARQSTSADFKDFD), which is not conserved in other mammalian PABPC proteins, was injected into rabbits for neuPABP antibody production (Thermo Fisher).

Sharma S., Kajjo S., Harra Z., Hasaj B., Delisle V., Ray D., Gutierrez R.L., Carrier I., Kleinman C., Morris Q., Hughes T.R., McInnes R, & Fabian M.R. (2023). Uncovering a mammalian neural-specific poly(A) binding protein with unique properties. Genes & Development, 37(15-16), 760-777.

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