Log In Sign up
The largest database of trusted experimental protocols

Pe cy7 conjugated il 4

Manufactured by Thermo Fisher Scientific
Visit Supplier

PE-Cy7-conjugated IL-4 is a fluorescently labeled cytokine protein used for the detection and analysis of interleukin-4 (IL-4) expression in biological samples. It can be used in various immunological and cell biology applications that involve the identification and quantification of IL-4-expressing cells.

Automatically generated - may contain errors

Lab products found in correlation

Golgiplug, by BD (2 mentions) Pe conjugated il 10, by Thermo Fisher Scientific (2 mentions) Apc conjugated il 17a, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

IL-4-PE (23 citations)

2 protocols using pe cy7 conjugated il 4

1

Comprehensive T Cell Cytokine Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
At the end of the 14-day culture, T cells were stimulated with Leukocyte activation cocktail with BD GolgiPlug (BD Biosciences, Cat. 550583) for 4 hours and washed in FACS buffer. BV650-conjugated CD3 (Clone OKT3) and PE-CF594-conjugated CD4 (Clone OKT4) antibody were used to detect these cell surface markers. We performed intracellular fixation and permeabilization with a commercial buffer set (eBiosciences, Cat. 88-8824). The following antibodies were used to detect intracellular cytokines: APC-conjugated IL-17A (Clone eBio64DEC17), FITC-conjugated IFNγ (Clone 4S.B3), PE-conjugated IL-10 (Clone JES3-9D7), and PE-Cy7-conjugated IL-4 (Clone 8D4-8) were purchased from eBioscience or Biolegend).
Chang Y.L., Rossetti M., Vlamakis H., Casero D., Sunga G., Harre N., Miller S., Humphries R., Stappenbeck T., Simpson K.W., Sartor R.B., Wu G., Lewis J., Bushman F., McGovern D.P., Salzman N., Borneman J., Xavier R., Huttenhower C, & Braun J. (2018). A screen of Crohn’s disease-associated microbial metabolites identifies ascorbate as a novel metabolic inhibitor of activated human T cells. Mucosal immunology, 12(2), 457-467.
+ Open protocol
+ Expand
2

Comprehensive T Cell Cytokine Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
At the end of the 14-day culture, T cells were stimulated with Leukocyte activation cocktail with BD GolgiPlug (BD Biosciences, Cat. 550583) for 4 hours and washed in FACS buffer. BV650-conjugated CD3 (Clone OKT3) and PE-CF594-conjugated CD4 (Clone OKT4) antibody were used to detect these cell surface markers. We performed intracellular fixation and permeabilization with a commercial buffer set (eBiosciences, Cat. 88-8824). The following antibodies were used to detect intracellular cytokines: APC-conjugated IL-17A (Clone eBio64DEC17), FITC-conjugated IFNγ (Clone 4S.B3), PE-conjugated IL-10 (Clone JES3-9D7), and PE-Cy7-conjugated IL-4 (Clone 8D4-8) were purchased from eBioscience or Biolegend).
Chang Y.L., Rossetti M., Vlamakis H., Casero D., Sunga G., Harre N., Miller S., Humphries R., Stappenbeck T., Simpson K.W., Sartor R.B., Wu G., Lewis J., Bushman F., McGovern D.P., Salzman N., Borneman J., Xavier R., Huttenhower C, & Braun J. (2018). A screen of Crohn’s disease-associated microbial metabolites identifies ascorbate as a novel metabolic inhibitor of activated human T cells. Mucosal immunology, 12(2), 457-467.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!