FISH was executed to determine the subcellular location of CASC18 in CAL27 cells. Fluorogenic probes targeting CASC18, 18 S and U6 were designed and synthesized by RiboBio (Guangzhou, China). Hybridization was carried out overnight according to the manufacturer’s instructions. All fluorescent images were captured with the same exposure setting and illumination.
Fluorogenic probes
Manufactured by RiboBio
Sourced in China
Fluorogenic probes are a type of lab equipment used to detect and quantify specific biomolecules in samples. These probes emit fluorescent signals upon binding or interacting with their target analytes, allowing for sensitive and real-time detection. The core function of fluorogenic probes is to serve as a tool for monitoring and analyzing biomolecular interactions and processes in research and diagnostic applications.
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Lab products found in correlation
2 protocols using fluorogenic probes
1
Subcellular Localization of CASC18 in CAL27 Cells
2
Subcellular Localization of CASC18 in SiHa Cells
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To determine the subcellular distribution of CASC18 in SiHa cells, fluorescence in situ hybridization (FISH) was performed using fluorogenic probes (RiboBio, Guangzhou, China) targeting CASC18, 18S, and U6 as described in our previous study.16 (link)
Briefly, cells were firstly fixed in 4% formaldehyde for 10 min. After washing with PBS, the fixed cells were then treated with 0.5% Triton X-100 in PBS for 5 min at 4°C. The specimens were then incubated with blocking solution for 30 min at 37°C. The hybridization was performed in the dark at 37°C overnight, followed by washing with gradient sodium citrate buffer at 42°C. Finally, the nucleus was stained with DAPI, and FISH specimens were examined under a fluorescence microscope (Motic, BA410E, China) to capture fluorescent images with the same exposure setting and illumination.
Briefly, cells were firstly fixed in 4% formaldehyde for 10 min. After washing with PBS, the fixed cells were then treated with 0.5% Triton X-100 in PBS for 5 min at 4°C. The specimens were then incubated with blocking solution for 30 min at 37°C. The hybridization was performed in the dark at 37°C overnight, followed by washing with gradient sodium citrate buffer at 42°C. Finally, the nucleus was stained with DAPI, and FISH specimens were examined under a fluorescence microscope (Motic, BA410E, China) to capture fluorescent images with the same exposure setting and illumination.
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