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2 protocols using ain93g mineral mix

1

Lipidomic Analysis Protocol

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The LC/MS-grade isopropanol, methanol, and chloroform were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). The mobile-phase additive ammonium acetate was purchased from Sigma-Aldrich (St. Louis, MO). The internal standards such as, phosphatidylethanolamine (PE 15:0-15:0), phosphatidylinositol (PI 8:0-8:0), phosphatidylcholine (PC 13:0-13:0), lysophosphatidylcholine (LPC) 15:0, lysophosphatidylethanolamine (LPE) 13:0, and lysophosphatidylinositol (LPI) 13:0, were obtained from Avanti Polar Lipids (Alabaster, AL). Triacylglyceride (TAG) 11:0-11:0-11:0 and fatty acid 17:0 were obtained from Sigma-Aldrich (St. Louis, MO). TK-16 (Dextran) from Mastutani Chemical Industry Co., Ltd. (Hyogo, Japan), NZMP acid casein from Fonterra Cooperative Group Ltd. (Auckland, New Zealand), sucrose from Nippon Beet Sugar Manufacturing Co., Ltd. (Tokyo, Japan), soybean oil from J-Oil Mills, Inc. (Tokyo, Japan), crystalline cellulose from Ceolus PH-102, Asahi Kasei Chemicals Corp., (Tokyo, Japan), AIN93G mineral mix from MP Biomedicals (USA), AIN-93VX vitamin mix from CLEA Japan Inc. (Tokyo, Japan), choline bitartrate from Wako Pure Chemical Industries Ltd. (Osaka, Japan), lard from Bell Shokuhin Co., Ltd. (Sapporo, Japan) and all other reagents of analytical grade were purchased from Kanto Chemical Co., Inc. (Tokyo, Japan) unless otherwise specified.
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2

Microbial Community Analysis Protocol

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Fructooligosaccharides (GFn, high performance liquid chromatography (HPLC) purity 95.93%) were obtained from Bao Lingbao Biotechnology Co., Ltd. (Shandong, China), the main components of which were 1F-fructofuranosylnystose (2.88%), nistose (32.45%), and 1-kestose (60.60%). AIN-93-VX Vitamin Mix and AIN-93G Mineral Mix were obtained from MP Biomedicals, LLC (Santa Ana, CA, USA).
FastDNA Spin Kit for Soil was obtained from Beijing Lianlixin BioTech Co., Ltd (Beijing, China). QIAquick Gel Extraction Kit was obtained from QIAGEN GmbH (Venlo, The Netherlands). Quanta iTico PicoGreen dsDNA Assay Kit was obtained from USA Life Technologies (Waltham, MA, USA). KAPA Biosystems Library Quantification Kit was obtained from KAPA Biosystems (Woburn, MA, USA). QubitTM dsDNA BR Assay Kit was obtained from Thermo Fisher Scientific (Waltham, MA, USA). TruSeq DNA LT Sample Preparation Kit and MiSeq Reagent Kit were obtained from Illumina Corporation (San Diego, CA, USA).
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