B cells were quantified using a research flow cytometry panel. Briefly, peripheral blood mononuclear cells (PBMCs) were incubated on ice for 30 minutes with fluorescently labeled antibodies (in FACS buffer containing DPBS and 1% newborn calf serum (Life Technologies)) prior to analysis on a FACSymphony (BD Bioscience) flow cytometer. The following antibodies were included for cell labelling: fixable viability dye (FV), anti-CD19 BV421 (HIB19, BD), anti-IgM FITC (G20-127, BD), anti-IgD BUV737 (IA6-2, BD), anti-CD27 PE-Cy7 (LG.7F9, Thermo Fisher), anti-CD45 BV510 (HI30, BD), anti-CD3 BV605 (UCHT1, BioLegend), anti-CD16 BV711 (3G8, BD), anti-CD14 BV711 (M0P-9, BD), anti-CD4 BUV395 (SK3, BD), and anti-CD8 PE (RPA-T8, BD). FlowJoTM Software version 10.7.1 (Ashland, OR) was used for analyses with flow cytometry proportions multiplied by absolute lymphocyte counts to calculate total CD19+ B cell numbers.
Anti cd3 bv605 ucht1
Manufactured by BioLegend
Anti-CD3 BV605 (UCHT1) is a fluorochrome-conjugated antibody that specifically binds to the CD3 receptor on human T cells. It is used as a tool in flow cytometry and other immunological applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd19 bv421 hib19, by BD (2 mentions)
Anti cd45 bv510 hi30, by BD (2 mentions)
Newborn calf serum, by Thermo Fisher Scientific (2 mentions)
Anti cd14 bv711 m0p 9, by BD (2 mentions)
Anti cd16 bv711 3g8, by BD (2 mentions)
Anti igm fitc g20 127, by BD (1 mentions)
Anti cd8 pe rpa t8, by BD (1 mentions)
Facsymphony, by BD (1 mentions)
Fixable viability stain 700, by BD (1 mentions)
Facsymphony a5, by BD (1 mentions)
2 protocols using anti cd3 bv605 ucht1
1
Quantifying B Cells via Flow Cytometry
2
Quantification of B Cells and Plasma Cells
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B cells were quantified using a research flow cytometry panel (Representative gates shown in Extended Data Fig. 8 ). Briefly, peripheral blood mononuclear cells (PBMCs) were incubated on ice for 30 minutes with fluorescently labeled antibodies (in fluorescence-activated single cell sorting [FACS] buffer containing Dulbecco's phosphate-buffered saline [DPBS] and 1% newborn calf serum [Life Technologies]) before its analysis on a FACSymphony A5 (BD Bioscience) flow cytometer. The following antibodies were included for cell labeling: fixable viability stain 700 (BD cat#564997), anti-CD19 BV421 (HIB19, BD), anti-CD45 BV510 (HI30, BD), anti-CD3 BV605 (UCHT1, BioLegend), anti-CD16 BV711 (3G8, BD), anti-CD14 BV711 (M0P-9, BD). FlowJoTM Software version 10.7.1 was used for analyses with flow cytometry proportions multiplied by absolute lymphocyte counts to calculate total CD19+ B cell numbers. Plasma cells in the bone marrow were quantified based on detection of CD138+ plasma cells in clinically obtained bone marrow core biopsies by immunohistochemistry in the University of Washington Hematopathology Laboratory.
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