Gt1 1

Mouse pituitary adenoma cell lines AtT-20 (CCL-89) was purchased from the American type culture collection (ATCC, USA) which has adrenocorticotropic hormone (ACTH) activity. Mouse pituitary adenoma cell lines GT1-1 was purchased from China Academy of Medical Sciences Cell Resource Centre, which has luteinising hormone-releasing hormone (LHRH) activity. Mouse pituitary cell MPC was purchased from ScienCell (USA). MPC and GT1-1 cells were cultured in high-glucose Dulbecco's modified eagle's medium (DMEM, Gibco, Life Technologies, Gaithersburg, MD, USA) supplemented with 10% horse serum (Gibco, Life Technologies, Gaithersburg, MD, USA) and 2.5% foetal bovine serum (FBS, Gibco, Life Technologies, Gaithersburg, MD, USA). AtT-20 cells were cultured in F-12K medium (Gibco, Life Technologies, Gaithersburg, MD, USA) supplemented with 10% horse serum (Gibco, Life Technologies, Gaithersburg, MD, USA) and 2.5% FBS (Gibco, Life Technologies, Gaithersburg, MD, USA). All of them were incubated at 37°C in a humidified atmosphere with 5% CO 2 .

Mouse pituitary adenomas cell lines AtT-20, GT1.1 and pituitary cell MPC were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). MPC cell and GT1.1 cell were cultured in high glucose Dulbecco's modified Eagle's medium (DMEM, Gibco, Life Technologies, Gaithersburg, MD, USA) supplemented with 10% horse serum (Gibco, Life Technologies, Gaithersburg, MD, USA) and 2.5% fetal bovine serum (FBS, Gibco, Life Technologies, Gaithersburg, MD, USA), AtT-20 cell was cultured in F-12K medium (Gibco, Life Technologies, Gaithersburg, MD, USA) supplemented with 10% horse serum (Gibco, Life Technologies, Gaithersburg, MD, USA) and 2.5% FBS (Gibco, Life Technologies, Gaithersburg, MD, USA). All of them were incubated at 37°C in a humidified atmosphere with 5% CO2.