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Cyclin a2

Manufactured by ABclonal
Sourced in United States, China

Cyclin A2 is a protein that plays a crucial role in cell cycle regulation. It is involved in the progression of the cell cycle from the G1 phase to the S phase and from the G2 phase to the M phase. Cyclin A2 acts as a regulatory subunit of cyclin-dependent kinases (CDKs), which are essential for the control of cell division and proliferation.

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2 protocols using cyclin a2

1

Osteoclastogenesis Pathway Molecular Assays

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The 12 ODNs, 2006, β-actin, Nfatc, c-fos, RANK, MMP9, and FAM labeled ODNs were synthesized by Takara (Dalian, China), and the sequences are listed in ESI Table 1. The PrimerScript® RT reagent kit and SYBR Green Premix Ex Taq kit were purchased from Takara. The antibodies (cyclin A2, cyclin B1, cyclin D1, cyclin E1, Nfatc, c-fos, RANK, MMP9, OPG, RANKL, β-actin, and horseradish peroxidase-conjugated goat anti-rabbit secondary antibodies) were all purchased from ABclonal (Boston, USA).The RAW264.7 cells (SCSP-5036) were purchased from Cell library of Chinese Academy of Sciences.
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2

Western Blot Analysis of Cell Signaling

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RIPA lysis buffer (#ab156034, Abcam, United Kingdom) was added to the liver and hepatocytes, and total proteins were extracted on ice with a glass tissue grinder (Kimble Chase, United States). Protein concentration was quantified using a BCA protein assay kit (Tiangen, China). Heat-denatured protein lysates (20 µg of protein) were separated by reducing SDS-PAGE electrophoresis and transferred to a PVDF membrane. The primary antibodies used had the following specificities: β-Actin (ABclonal, AC004, China), ATM (ABclonal, A18257, China), Chk-2 (ABclonal, A2145, China), P53 (ABclonal, A0263, China), Cyclin D1 (ABclonal, A11310, China), Cyclin E1 (ABclonal, A14225, China), Cyclin A2 (ABclonal, A2891, China), and Cyclin B1 (ABclonal, A2056, China). The secondary antibodies used were the following: goat anti-rabbit (IgG H + L: ABclonal, AS003, China) and goat anti-mouse (IgG H + L: ABclonal, AS014, China). The bands were visualized by adding electrochemiluminescence (ECL) reagents (GoodHere, China) and with a luminescent image analyzer (Fujifilm LAS-3000, Japan). Protein levels were quantified using Image J software (National Institutes of Health, United States).
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