TentaGel S Resin was purchased from Rapp Polymere (Germany, loading 0.31 mmol/g). 9-Fluorenylmethoxycarbonyl (Fmoc)-protected amino acids, 2-(1H-benzotriazole-1-yl)-1,1,3,3- tetramethyluronium hexafluoro-phosphate (HBTU), and Wang resin were obtained from GL Biochem (China). Trifluoroacetic acid (TFA), fluorescein isothiocyanate (FITC), N-methyl morpholine (NMM), piperidine, and N,N′-dimethylformamide (DMF) were all from Beijing Chemical Plant (China). 1,2-Ethanedithiol (EDT) was purchased from Alfa Aesar (USA). Dimethyl sulfoxide (DMSO) was purchased from Aldrich Chemical Co. and used without further purification. Cyanogen bromide (CNBr) was from J&K Chemical (China). The HeLa cell lines were received from the Cell Culture Center of the Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (Beijing, China). Cell culture medium and fetal bovine serum were from WisentInc (Multicell, WisentInc, St. Bruno. The cell counting kit-8 assay (CCK-8) (Beyotime Institute of Biotechnology, China) was used. Hela cells were maintained Dulbecco’s modified eagle’s medium (DMEM) with 10% fetal bovine serum and 1% penicillin. All cells were cultured in a humidified atmosphere containing 5% CO2 at 37 °C.
9 fluorenylmethoxycarbonyl fmoc protected amino acids
Manufactured by GL Biochem
Sourced in China
9-Fluorenylmethoxycarbonyl (Fmoc)-protected amino acids are a class of amino acid derivatives commonly used in solid-phase peptide synthesis. They serve as building blocks for the construction of peptide and protein molecules. Fmoc-protected amino acids provide a temporary protecting group that allows for the controlled addition of individual amino acid residues during the synthesis process.
Automatically generated - may contain errors
Lab products found in correlation
2 1h benzotriazole 1 yl 1 1 3 3 tetramethyluronium hexafluorophosphate hbtu, by GL Biochem (2 mentions)
N n dimethylformamide (dmf), by Beijing Chemical Works (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
N methyl morpholine, by Beijing Chemical Works (1 mentions)
1 2 ethanedithiol edt, by Thermo Fisher Scientific (1 mentions)
Wang resin, by GL Biochem (1 mentions)
Cell counting kit 8 cck 8 assay, by Beyotime (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Fluorescein 5 isothiocyanate, by Merck Group (1 mentions)
Hplc system l 7100, by Hitachi (1 mentions)
Trifluoroacetic acid tfa, by Merck Group (1 mentions)
Maldi tof ms, by Bruker (1 mentions)
Milli q water, by Merck Group (1 mentions)
1 2 dipalmitoyl sn glycero 3 phosphocholine dppc, by Avanti Polar Lipids (1 mentions)
1 2 dipalmitoyl sn glycero 3 phospho 1 rac glycerol, by Avanti Polar Lipids (1 mentions)
3 protocols using 9 fluorenylmethoxycarbonyl fmoc protected amino acids
1
Peptide Synthesis and Cell Viability Assay
2
Peptide Synthesis and Purification Protocol
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All peptides were synthesized using Fmoc strategy SPPS (solid phase peptide synthesis). The synthesized peptides were purified by using a Hitachi HPLC system (L-7100, Japan) on a TSK gel ODS-100V column (150 mm×4.6 mm) at a flow rate of 2 mL min-1. Gradient: 0-25 min, 5-80% acetonitrile containing 0.1% Trifluoroacetic acid (TFA). After purification, peptides were characterized by MALDI-TOF MS (Bruker Daltonics).
9-Fluorenylmethoxycarbonyl (Fmoc)-protected amino acids and 2-(1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HBTU) were purchased from GL Biochem (China). Trifluoroacetic acid (TFA), and fluorescein 5-isothiocyanate were from Sigma-Aldrich (USA). N-Methyl morpholine (NMM) and N,N-dimethylformamide (DMF) were from Beijing chemical plant (China).
9-Fluorenylmethoxycarbonyl (Fmoc)-protected amino acids and 2-(1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HBTU) were purchased from GL Biochem (China). Trifluoroacetic acid (TFA), and fluorescein 5-isothiocyanate were from Sigma-Aldrich (USA). N-Methyl morpholine (NMM) and N,N-dimethylformamide (DMF) were from Beijing chemical plant (China).
3
Peptide Synthesis and Purification Protocol
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The peptides were synthesized by Fmoc solid-phase synthesis using a commercial CEM Liberty peptide synthesizer. The synthesis was carried out from the C-terminus to the N-terminus on the Rink amide MBHA resin, thus producing C-terminally amidated peptides. The peptides were purified by cold ether precipitation eight times to reach the purity of >95%, followed by lyophilization for 2 days. The peptide solutions were prepared by dissolving the lyophilized peptide powders in Milli-Q water (Millipore Reagent Water System, USA) and their pH was adjusted to the desirable range using sodium hydroxide. All the chemicals, reagents and organic solvents were sourced from Merck (Sigma Aldrich), UK, with analytical grade. Rink amide-methylbenzhydrylamine hydrochloride salt (MBHA) resin, and 9-fluorenyl-methoxycarbonyl (Fmoc) protected amino acids were bought from GL Biochem Ltd (Shanghai, China).1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2dipalmitoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (DPPG) were purchased from Avanti Polar Lipids (USA). The Dulbecco's Modified Eagle Medium (DMEM), Phosphate buffered saline (PBS), Fetal bovine serum (FBS), Trypsin, Penicillin and streptomycin were sourced from GIBCO (Thermo Fisher Scientific, UK). JC-1 mitochondrial probe (Invitrogen™) was sourced from Invitrogen (Thermo Fisher Scientific, UK).
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