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Xf cell glycolysis test kit

Manufactured by Agilent Technologies
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The XF Cell Glycolysis Test Kit is a lab equipment product from Agilent Technologies that measures the extracellular acidification rate (ECAR) of cells. This measurement provides insights into the glycolytic activity of the cells under study.

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Lab products found in correlation

Non buffered assay medium, by Agilent Technologies (1 mentions) Xf24 extracellular flux analyzer, by Agilent Technologies (1 mentions)

Spelling variants of this product

Seahorse XF Cell Glycolysis Stress Test Kit (7 citations) XF cell glycolysis stress test kit (3 citations)

2 protocols using xf cell glycolysis test kit

1

Measuring Glycolytic Flux in A-549 Cells

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The extracellular acidification rate (ECAR) was measured using an XF Cell Glycolysis Test Kit (Agilent Technologies, Inc., USA). Briefly, approximately 5 × 104 A-549 cells per well were seeded onto XF24 microplates and incubated for 60 min in a non-buffered assay medium without CO2. The recording began by establishing baseline ECAR values followed by sequential injections of 10mM of glucose, 1 μM of oligomycin, or 50 mM of 2-deoxy-D-glucose (2-DG).
Ji X., Zhu R., Gao C., Xie H., Gong X, & Luo J. (2024). Hypoxia-Derived Exosomes Promote Lung Adenocarcinoma by Regulating HS3ST1-GPC4-Mediated Glycolysis. Cancers, 16(4), 695.
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2

Extracellular Acidification Rate Assay

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Extracellular acidification rate (ECAR) was determined using a seahorse XF24 Extracellular Flux Analyzer (Agilent Seahorse Bioscience) following the manufacturer’s protocols. A total of 5 × 104 cells were plated in each well of the XF24 microplates. Cells were maintained in a non-buffered assay medium (Agilent) in a non-CO2 incubator for 60 min before the assay. The XF Cell glycolysis Test Kit (Agilent, 103020) was used for the assay. The baseline recordings were followed by sequential injection of the following reagents: glucose (10 mM), oligomycin (1 μM), or 2-DG (50 mM).
Lin S., Li Y., Wang D., Huang C., Marino D., Bollt O., Wu C., Taylor M.D., Li W., DeNicola G.M., Hao J., Singh P.K, & Yang S. (2021). Fascin Promotes Lung Cancer Growth and Metastasis by Enhancing Glycolysis and PFKFB3 Expression. Cancer letters, 518, 230-242.
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