Following identification by matrix-assisted laser desorption ionization-time of flight mass spectrometry, S. aureus isolates were subjected to antimicrobial susceptibility testing to determine their antibiotic resistance phenotypes. Each bacterial colony was resuspended in 0.45% sodium chloride (bioMérieux) by using sterile swabs to prepare 0.5-McFarland suspensions, in accordance with the manufacturer’s recommendations. Subsequently, 280 µL of bacterial suspension was diluted in 3 mL of 0.45% sodium chloride and transferred into Vitek-2 AST-GP67 cards, which were then loaded into the Vitek-2 Compact system (bioMérieux) for automatic incubation and reporting. Raw minimum inhibitory concentrations (μg/mL) were converted to sensitive, intermediate, and resistant classifications, in accordance with the 2018 guidelines from the Clinical and Laboratory Standard Institute.14 S. aureus ATCC 25923 was used as a quality control for antimicrobial susceptibility testing.
Sodium chloride (nacl)
Manufactured by bioMérieux
Sourced in France
Sodium chloride is a chemical compound that is a natural mineral and a major component of salt. It is a white, crystalline solid that is commonly used in laboratory settings as a reference standard or as a component in various laboratory procedures.
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Lab products found in correlation
Vitek 2 ast gp 67 cards, by bioMérieux (1 mentions)
Vitek 2 compact system, by bioMérieux (1 mentions)
Gallocatechin, by Merck Group (1 mentions)
Vanillic acid, by Thermo Fisher Scientific (1 mentions)
Naringenin, by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline (pbs), by bioMérieux (1 mentions)
Quercetin, by Merck Group (1 mentions)
2 protocols using sodium chloride (nacl)
1
Antimicrobial Resistance Profiling of S. aureus
2
Tannin Extraction from Diverse Plants
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Arbutin, luteolin and taxifolin were purchased from Apin Chemicals Ltd (Abingdon, U.K.); vanillic acid and naringenin from Alfa Aesar (Blackpool, U.K.); gallocatechin and quercetin from Sigma–Aldrich (Gillingham, U.K.); phosphate buffered saline (PBS) from bioMerieux (Lyon, France), Milton solution, sodium hypochlorite 2% w/v and sodium chloride 16.5% w/v from Milton (Inibsa laboratorios, Barcelona, Spain). Goat willow (Salix caprea), black currant (Ribes nigrum) and red currant (Ribes rubrum) leaves were collected in July and August 2012. Tilia (Linden) flowers (Tilia × europaea) were purchased from Flos (Mokrsko, Poland). These plant samples were chosen as they allowed the testing of different tannin types, all of which are known to occur in willow.
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