Peroxidase goat anti rabbit or mouse h l

Rabbit anti-ERLI (UM510) and Rabbit anti-CLPI (UM377, 1:250–500) were a kind gift from Dr Margolis, University of Michigan, USA).
Rabbit anti-pan-Crb3 (C2, kind gift from Dr A.Le Bivic, IBDML, France, 1:250)
Rabbit anti-YAP (#4912, Cell Signaling, 1:1,000)
Rabbit anti-phospho-YAP (S127A) (#4911, Cell Signaling, 1:1,000)
Rabbit anti-GFP (A11122, Invitrogen, 1:500)
Mouse anti-FLAG (F-3165, Sigma, 1:500)
Rabbit anti-MAG (#9086, Cell Signaling, 1:1,000)
Goat anti-MPZ (PA5-18773, Thermo Fisher, 1:3,000)
Rabbit anti-Rab11a (#2413, Cell Signaling, 1:1,000)
Rabbit anti-lamininγ1 (NBP1-19643, Novus Biologicals, 1:500)
Mouse anti-MBP (SMI94, Sternberger Monoclonal incorporated, 1:1,000)
Mouse anti-α-β-Actin (C1.AC-15, #A1978, Sigma Aldrich, 1:10,000)
Rabbit Pan-TEAD (D3F7L, Cell Signaling, 1:1,000)
Mouse anti-AKT (Cell signaling, 1:1,000)
Mouse anti-P-AKT (ser473, 587F11, Cell signaling, 1:1,000)
Secondary antibodies, Peroxidase Goat anti Rabbit or Mouse (H+L) (Jackson Immuno Research) were used at 1:10,000 dilution.

Sciatic nerves were dissected from mice, after removal of the epineurium and perineurium, the nerves were frozen in liquid nitrogen and conserved at -80°C. Protein were extracted from whole sciatic nerves after homogenization by sonication in standard RIPA lysis buffer and the protein concentration was measured using a BCA protein assay kit (Pierce). 20 μg proteins were directly analyzed by western blot using standard procedures with 12% SDS–PAGE and transferred on PVDF membranes for immunoblotting. Primary antibodies: Rabbit anti-Pkm1 1/2500 (NBP2-14833, Novus Biologicals); Rabbit anti-Pkm2 1/2500 (SAB4200095, Sigma Aldrich); Mouse anti-α-β-Actin 1/10000 (C1.AC-15, #A1978, Sigma Aldrich). Secondary antibodies, Peroxidase Goat anti Rabbit or Mouse (H+L) (Jackson Immuno Research) were used at 1/10000 dilution.