Pparδ transcription factor assay kit

DHA (cat # 90310), N-methylsulphonyl-6-(2-proparglyloxyphenyl) hexanamide (MS-PPOH) (cat # 75770) and 19, 20 – EDP (cat # 10175) were purchased from Cayman Chemicals (Ann Arbor, MI, USA). Tissue culture materials were obtained from Invitrogen (Burlingt on, ON, Canada); Bradford protein assay solution from Bio-Rad Laboratories (Mississauga, ON, Canada); Primary and secondary antibodies from Cell Signaling (Pickering, ON, Canada); Trypan blue, MTT, 2′, 7′-Dichlorofluorescin diacetate, myriocin from Sigma–Aldrich (Oakville, ON, Canada). Annexin V-FITC and propidium iodide from BDsciences; Caspase 3 assay kit from Sigma (Saint Louis, MO, USA cat# CASPC3C-1KT); Total 20S proteasome assay kit from Chemicon (EMD Millipore, Etobicoke, ON, Canada cat # APT280); Lipid peroxidation (MDA) cat# ab118970; PPARδ transcription factor assay kit Cayman Chemicals (Ann Arbor, MI, USA cat # 90,310). GSK 3787 cat# 3961; Ceramide cat# 0744 from Tocris bioscience (Bristol, UK). Cell Counting Kit-8 (CCK-8) from Dojindo Molecular Technologies Inc. (Burlington, ON, Canada) cat# CK04–05. EDP methyl esters regioisomer mixtures (1:1:1:1:1:1; 4,5-, 7,8-, 10,11-, 13,14-, 16,17- and 19,20-EDP) were prepared as described previously (Morisseau et al., 2010 (link)).

PPARδ Transcription Factor Assay Kit (10006914, Cayman Chemical) was used according to the manufacturer’s instructions, to assess PPARδ’s DNA binding activity.