S6k1 sc 8418

Manufactured by Santa Cruz Biotechnology

Sourced in United States

S6K1 (sc-8418) is a protein-specific primary antibody produced in mouse. It recognizes the S6K1 (p70 S6 kinase 1) protein, which plays a role in the regulation of cell growth and proliferation.

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Lab products found in correlation

Phlpp1 a300 660a, by Fortis Life Sciences (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Dykddddk tag, by Cell Signaling Technology (1 mentions) Myc tag, by Cell Signaling Technology (1 mentions) Ha tag, by Cell Signaling Technology (1 mentions) Polyvinylidene difluoride membrane, by Merck Group (1 mentions) Ripa buffer, by Solarbio (1 mentions) Bca protein assay kit, by Solabio (1 mentions) P27 25614 1 ap, by Proteintech (1 mentions) Gapdh 10494 1 ap, by Proteintech (1 mentions) Cyclin d1 26 939 1 ap, by Proteintech (1 mentions)

Close spelling variants of this product

Sc-8418 (5 citations)

2 protocols using s6k1 sc 8418

Immunoblotting Analysis of Signaling Pathways

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Immunoblots were conducted on 3–7 samples randomly chosen within each experimental cohort with antibodies against Akt (#9272), p-Akt (S473, #4060), p-Akt (T308, #13038), p-S6K1 (T389, #9205), p-S6 (S240/244, #2215), S6 (#2217), p-(Ser/Thr) PKA substrate (#9621), PKA (#4782), DYKDDDDK-tag (#14793), HA-tag (#3724), myc-tag (#2276), and β-actin (#4970) from Cell Signaling; S6K1 (sc-8418) from Santa Cruz; and PHLPP1 (A300-660A) and PHLPP2 (A300-661A) from Bethyl Laboratories, Inc. Antibodies directed to human p-PHLPP2 (S1119 or S1210, identical to S1116 or S1207 in mouse PHLPP2) were generated by immunizing rabbits with synthetic phospho-specific peptides, verified by ELISA and affinity purified (Lifetein).

Kim K., Ryu D., Dongiovanni P., Ozcan L., Nayak S., Ueberheide B., Valenti L., Auwerx J, & Pajvani U.B. (2017). Degradation of PHLPP2 by KCTD17, via a Glucagon-dependent Pathway, Promotes Hepatic Steatosis. Gastroenterology, 153(6), 1568-1580.e10.

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Cell Lysis and Protein Immunoblotting

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Cells were collected in RIPA buffer (Solarbio, China) with phenylmethylsulfonylfluoride (PMSF) and phosphatase cocktails (Thermo Scientific). The concentration of the total protein of the above lysates was detected using the BCA Protein Assay Kit (Solabio, China). Total protein (20–30μg) was added to each mini pool of SDS-PAGE gels, separated at 80 V for 2 hours and transferred to a polyvinylidene difluoride membrane (Millipore, USA) at 200 mA for 2 hours.
Primary antibodies used in the whole experiment are listed below: ACTL6A (NB100-61628) from Novus Biologicals (USA); p21 (#2946), CDK2 (#18048), S6 (#2217) and pS6 (#5364) from Cell Signaling Technology (USA); S6K1 (sc-8418) from Santa Cruz; GAPDH (10494-1-AP), cyclinD1 (26939-1-AP) and p27 (25614-1-AP) from Proteintech (Wuhan, China).

Li R.Z., Li Y.Y., Qin H, & Li S.S. (2021). ACTL6A Promotes the Proliferation of Esophageal Squamous Cell Carcinoma Cells and Correlates with Poor Clinical Outcomes. OncoTargets and therapy, 14, 199-211.

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