Terbinafine hcl

Amorolfine HCl was purchased from Ranbaxy Research Laboratories (Haryana, India) and terbinafine HCl from AK Scientific (CA, USA). Diurethane dimethacrylate, EMA, HEMA, 2hydroxy-2-methylpropiophenone, absolute ethanol, methanol, mercaptoethanol, NMP, propan-2-ol, triethylamine, phosphoric acid 85% wt. solution in water and trifluoroacetic acid were purchased from Sigma-Aldrich (Dorset, UK). Acetonitrile HPLC gradient grade and PEG 200 were purchased from Fisher Scientific (Hertfordshire, UK and Loughborough, UK respectively). A 36 W Cuccio Professional UVA nail lamp was purchased from Amazon UK and a 36 W Professional Salon Quality 18 K UV-LED nail lamp was purchased from eBay UK. Nail & Beauty Emporium super absorbent lint-free wipes (4 ply) were purchased from Just Beauty UK, an online retailer specialising in professional beauty, hair and skin products. Healthy human nail clippings (fingernails) were obtained from male and female volunteers aged between 18 and 65 years following ethics approval (REC/B/10/01 School of Pharmacy, University of London, UK).

Amorolfine HCl was purchased from Ranbaxy Research Laboratories (Haryana, India) and terbinafine HCl from AK Scientific (CA, USA). Diurethane dimethacrylate, ethyl methacrylate, isobornyl methacrylate, 2-hydroxyethyl methacrylate, 2-hydroxy-2-methylpropiophenone, absolute ethanol, methanol, propan-2-ol, sodium chloride, triethylamine, phosphoric acid 85% wt solution in water and trifluoroacetic acid and a dialysis tubing cellulose membrane (MW 10281) were purchased from Sigma-Aldrich (Dorset, UK). Acetonitrile HPLC gradient grade was purchased from Fisher Scientific (Hertfordshire, UK). A 36 Watt Cuccio Professional UVA nail lamp was purchased from Amazon UK. Nail & Beauty Emporium lintfree wipes (4 ply) were purchased from Just Beauty UK, an online retailer specialising in professional beauty, hair and skin products. Sabouraud dextrose agar was purchased from Oxoid Ltd (Basingstoke, UK). One dermatophyte strain, Trichophyton rubrum (CBS 118892), was used. Curanail® nail lacquer was used as a control in some experiments and was purchased from pharmacies in the UK. Human nail clippings (fingernails) were obtained from healthy volunteers aged between 18 and 65 years (following ethics approval).