1 2 dioleoyl sn glycero 3 phosphoethanolamine n carboxyfluorescein

Anti-microRNA103/107 (5′-rUrCrArUrArGrCrCrCrUrGrUrArCrArArUrGrCrUrGrCrU-3′) and (5′-/5RhoR-XN/rUrCrArUrArGrCrCrCrUrGrUrArCrArArUrGrCrUrGrCrU-3′), namely anti-miR103/107, was synthesized by Tema Ricerca s.r.l. (Bologna, Italy). 1,2-dioleyl-3-dimethylammonium propane (DODAP), N-palmitoyl-sphingosine-1-succinyl[methoxy(polyethylene glycol)2000] (PEG2000-Cer16) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000]-Maleimide (DSPE-PEG-Mal) and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(carboxyfluorescein) (ammonium salt) were obtained by Avanti Polar Lipids. Disteroylphosphatidylcholine (DSPC) was kindly offered from Lipoid GmbH (Cam, Switzerland). Cholesterol (CHOL), human transferrin (Tf), ammonium ferrithiocyanate, sodium borate, sodium chloride, sodium citrate, sodium phosphate, HEPES, ethylenediaminetetraacetic acid (EDTA), citric acid, Sepharose G-25, and 2-iminothiolane (Traut’s reagent) were purchased from Sigma-Aldrich (USA). Ethanol and other solvents were obtained by Exacta Optech (Italy).

Senicapoc was a gift from Prof. Heike Wulff, University of California, Davis. Dipalmitoylphosphatidylcholine (DPPC) and 1,2-Dioleoyl-sn-Glycero-3-Phosphoethanolamine-N-(Carboxyfluorescein) were purchased from Avanti Polar Lipids (Abalaster, AL, USA). Whatmann drain discs and polycarbonate membrane filters were purchased from GE Healthcare Life Sciences (Freiburg, Germany). Chloroform (HPLC Grade) was purchased from Tedia Chemicals (Fairfield, OH, USA). Acetonitrile and Methanol (LCMS Grades) were purchased from Fisher Scientific (Hampton, NH, USA). Cellulose ester dialysis tubings (100 kDa MWCO) and tubing closures were obtained from Spectrum Laboratories (Rancho Dominguez, CA, USA). Salts used to make Phosphate Buffered Saline (PBS) includes sodium chloride (NaCl), potassium chloride (KCl), Di-sodium hydrogen phosphate (Na₂HPO₄) and Potassium dihydrogen phosphate (KH₂PO₄) were all purchased from Sigma-Aldrich (St. Louis, MO, USA). Zinc Sulphate Monohydrate salt for analytical processing was also purchased from Sigma-Aldrich. Soft tissue homogenizing kits (CK14, 0.5 mL) used for tissue analysis were purchased from Bertin-Instruments (Rockville, MD, USA).