Log In Sign up
The largest database of trusted experimental protocols

Human il 18 elisa kit

Manufactured by MBL Life Science
Visit Supplier
Sourced in United States

The Human IL-18 ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human interleukin-18 (IL-18) levels in biological samples. The kit utilizes a pre-coated microplate with specific antibodies and provides the necessary reagents to perform the assay.

Automatically generated - may contain errors

Lab products found in correlation

Human tnf α quantikine elisa kit, by R&D Systems (1 mentions) Human ccl2 mcp 1 quantikine elisa kit, by R&D Systems (1 mentions) Quantikine elisa kit, by R&D Systems (1 mentions) Quantikine human il 1β il 1f2 immunoassay, by R&D Systems (1 mentions) Human il 8 duoset elisa kit, by R&D Systems (1 mentions) Human il 1β duoset elisa kit, by R&D Systems (1 mentions) Glucose assay kit, by Cell Biolabs (1 mentions) Fluorometric lactate assay kit, by Cell Biolabs (1 mentions) Human il 6 quantikine elisa, by R&D Systems (1 mentions) Pierce ldh cytotoxicity assay kit, by Thermo Fisher Scientific (1 mentions) Il 1 beta human uncoated elisa kit, by Thermo Fisher Scientific (1 mentions) Opteia elisa set, by BD (1 mentions) Softmax pro version 5, by Molecular Devices (1 mentions) Versamax microplate reader, by Molecular Devices (1 mentions) Purelink rna mini kit, by Thermo Fisher Scientific (1 mentions) Human il 18bpa duoset, by R&D Systems (1 mentions) Superscript 2 reverse transcriptase, by Thermo Fisher Scientific (1 mentions) Taqman gene expression assay, by Thermo Fisher Scientific (1 mentions) Rneasy micro kit, by Qiagen (1 mentions)

6 protocols using human il 18 elisa kit

1

Cytokine Profiling from Serum Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Blood samples for cytokine measurements were centrifuged for 10 min at approximately 3000 r/min 4 °C, and the serum was separated and stored at − 80 °C prior to analysis. We measured seven circulating serum cytokine levels. Circulating levels of receptor activator of nuclear factor-kappa B ligand (RANKL), adiponectin, monocyte chemotactic protein-1 (MCP-1), interleukin-6 (IL-6), human pentraxin 3 (PTX3), interleukin-18 (IL-18), and tumor necrosis factor-α (TNF-α) were determined by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer's instructions. ELISA Kit for receptor activator of nuclear factor kappa B ligand (USCN Life Science Inc., Houston, TX), human total adiponectin/Acrp30 Quantikine ELISA Kit, human CCL2/MCP-1 Quantikine ELISA Kit, human interleukin-6 (IL-6) Quantikine ELISA Kit, human pentraxin 3/TSG-14 Quantikine ELISA Kit, human TNF-α Quantikine ELISA Kit (R&D Systems, Inc., Minneapolis, MN), and human IL-18 ELISA Kit (MBL International, Woburn, MA) were used.
Namba S., Yamaoka-Tojo M., Hashikata T., Ikeda Y., Kitasato L., Hashimoto T., Shimohama T., Tojo T., Takahira N., Masuda T, & Ako J. (2015). Long-term warfarin therapy and biomarkers for osteoporosis and atherosclerosis. BBA Clinical, 4, 76-80.
+ Open protocol
+ Expand
2

Quantifying IL-1β and IL-18 in Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols
The Quantikine Human IL-1β/IL-1f2 Immunoassay (R&D Systems, Minneapolis, MN, measures predominantly mature IL-1β) was used on concentrated cell medium, prepared as described earlier, and the assay performed according to the manufacturer’s instructions as previously published [3 (link)]. IL-18 release was measured using the Human IL-18 ELISA kit (MBL International, Woburn, MA, measures predominantly active IL-18, 0.7% pro IL-18). Values were expressed as pg/mL of IL-1β or IL-18 from the original supernatant (non-concentrated).
Westbom C., Thompson J.K., Leggett A., MacPherson M., Beuschel S., Pass H., Vacek P, & Shukla A. (2015). Inflammasome Modulation by Chemotherapeutics in Malignant Mesothelioma. PLoS ONE, 10(12), e0145404.
+ Open protocol
+ Expand
3

Cytokine Quantification in Supernatants

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cytokines were quantified in supernatants using human IL-1β DuoSet ELISA kit (DY201, R&D systems), human IL-18 ELISA Kit (7620, MBL International), human IL-8 DuoSet ELISA kit (DY208, R&D systems), and human MCP-1 DuoSet ELISA kit (DY279, R&D systems), according to the manufacturers’ instructions.
Shi H., Gandhi A.A., Smith S.A., Wang Q., Chiang D., Yalavarthi S., Ali R.A., Liu C., Sule G., Tsou P.S., Zuo Y., Kanthi Y., Farkash E.A., Lin J.D., Morrissey J.H, & Knight J.S. (2021). Endothelium-protective, histone-neutralizing properties of the polyanionic agent defibrotide. JCI Insight, 6(17), e149149.
+ Open protocol
+ Expand
4

Cytokine and Metabolic Profiling of CF Cultures

Check if the same lab product or an alternative is used in the 5 most similar protocols
Conditioned supernatants of human CF cultures were snapfrozen at -80 °C and assessed by colorimetric/fluorometric assay or ELISA using the following kits as instructed by the manufacturer: Pierce® LDH Cytotoxicity Assay Kit and Human IL-1beta Uncoated ELISA Kit (both from Thermo Fisher), Glucose Assay Kit and Fluorometric Lactate Assay Kit (both from Cell Biolabs, San Diego, CA, USA), Human IL-6 Quantikine ELISA (R&D Systems, Wiesbaden, Germany), Human IL-18 ELISA Kit (MBL International, via Biozol, Eching, Germany).
Mann C., van Alst C., Gorressen S., Nega R., Dobrev D., Grandoch M, & Fender A.C. (2024). Ischemia does not provoke the full immune training repertoire in human cardiac fibroblasts. Naunyn-Schmiedeberg's archives of pharmacology.
+ Open protocol
+ Expand
5

Cytokine Production by Stimulated MoDCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Supernatants from MoDCs stimulated for 6, 18, 24 or 72 hours in a humidified incubator (37°C, 5% CO2) with TLRAs, and/or CLRAs were analyzed for TNF, IL-18 or IL-23 production. For this, human enzyme-linked immunosorbent assay (ELISA) kits were used as per the manufacturer’s instruction. TNF: BD Opteia™ ELISA set (BD Biosciences; San Jose, CA, USA). IL-23: Human IL-23 Quanktikine ® ELISA kit (R&D systems; Minneapolis, MN, USA). IL-18: Human IL-18 ELISA kit (MBL International: Woburn, MA, USA). ELISA plates were read on a Versamax microplate reader with SoftMax Pro Version 5 (both from Molecular Devices; Sunnyville, CA, USA).
van Haren S.D., Dowling D.J., Foppen W., Christensen D., Andersen P., Reed S.G., Hershberg R.M., Baden L.R, & Levy O. (2016). Age-specific Adjuvant Synergy: Dual TLR7/8 and Mincle Activation of Human Newborn Dendritic Cells Enables Th1-polarization. Journal of immunology (Baltimore, Md. : 1950), 197(11), 4413-4424.
+ Open protocol
+ Expand
6

RNA Isolation, cDNA Synthesis, and Cytokine Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA was purified using the RNeasy Micro kit (Qiagen, Hilden, Germany) for patient material or the PureLink RNA mini kit (Invitrogen, Carlsbad, CA, USA). cDNA synthesis was performed using Super Script II Reverse Transcriptase (Invitrogen), according to manufacturer's protocols. mRNA levels were analyzed in duplicate by qPCR using TaqMan Gene Expression Assays [Applied Biosystems, Foster City, CA, USA; Assay IDs: Hs00171042_m1 (IP-10/CXCL10), Hs00158032_m1 (IDO), Hs00271720_m1 (IL-18BP), Hs01555410_m1 (IL-1β), Hs00174103_m1 (IL-8/CXCL8)] and normalized to housekeeping gene GAPDH. Cytokine levels in plasma were measured by sandwich ELISA according to the manufacturer's protocols (IFN-γ, IL-6, IL-1β High Sensitivity ELISA, eBioscience, San Diego, CA, USA; Human IL-18 ELISA kit MBL International, Woburn, MA, USA; Human IL-18BPa Duoset, R&D Systems, Minneapolis, MN, USA). IP-10, MIG and IL-8 levels were quantified by sandwich ELISA as described [37, 38] . Levels of free IL-18 were calculated as described [39] .
Put K., Avau A., Brisse E., Mitera T., Put S., Proost P., Bader-Meunier B., Westhovens R., Van den Eynde B.J., Orabona C., Fallarino F., De Somer L., Tousseyn T., Quartier P., Wouters C, & Matthys P. (2015). Cytokines in systemic juvenile idiopathic arthritis and haemophagocytic lymphohistiocytosis: tipping the balance between interleukin-18 and interferon-γ. Rheumatology (Oxford, England), 54(8).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!