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Anti ubiquitin

Manufactured by Fortrea

Anti-ubiquitin is a lab equipment product designed to detect and analyze ubiquitin proteins. Ubiquitin is a small regulatory protein found in most tissues of eukaryotic organisms and plays a crucial role in various cellular processes. The anti-ubiquitin product provides researchers with a tool to identify and study the presence and function of ubiquitin in biological samples.

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2 protocols using anti ubiquitin

1

Antibody-Based Protein Detection Protocol

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The antibodies used are as follows: anti-Myc (Bio Legend-626802), anti-FLAG-HRP (Roche-6952, Sigma-A8592), anti-FLAG (Sigma-F3165), anti-FLAG-HRP (Roche-6952), anti-Cse4 (polyclonal rabbit antibody against recombinant Cse4), anti-H2A (Active Motif-39235), anti-Pgk1 (Invitrogen-459250), anti-ubiquitin (Covance-MMS257P), anti-HA (Covance-PRB101P), anti-FLAG beads (Sigma-F2426), secondary antibodies were HRP linked, anti-rabbit IgG (from donkey) and anti-mouse IgG (from sheep) (GE Healthcare, NA934V and NA931V, respectively).
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2

Antibodies for Molecular Biology Assays

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LMS cells were grown as previously described [28 (link)]. Primary antibodies used and reagents were: anti-SKP2 8D9 (Life Technologies); anti-MEF2C [35 (link)] anti-MEF2D α1/α2 [10 (link)]; anti-GFP, anti-HDAC4 [60 (link)] and anti-HDAC5 [38 (link)]; anti-HDAC7 (sc-74563), anti-MEF2A (C-21 sc-313) and anti-RACK1 (sc-17754, Santa Cruz Biotechnology); anti-MEF2D (BD Transduction Laboratories); anti-Actin, p21 CP74 and FLAG M2 (Sigma-Aldrich); anti-ubiquitin (Covance); anti-HDAC9 (ab109446), anti-H3K27ac (ab4729) and anti-H3K27me3 (ab6002) (Abcam); anti-H3K4me3 (GTX128954, GeneTex); anti-KI67 (556003, BD Pharmingen).
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