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Biozero

Manufactured by Keyence
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Sourced in Japan, Germany

The Biozero is a piece of lab equipment designed for biological sample analysis. It provides core functions for sample preparation and data collection, enabling researchers to conduct various biological experiments and studies.

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Lab products found in correlation

Bz analyzer, by Keyence (3 mentions) Matrigel, by Corning (2 mentions) Diur 100, by BioAssay Systems (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Alexa 488 conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) α sma, by Abcam (1 mentions) Tsa blocking reagent, by PerkinElmer (1 mentions) Fv300 laser confocal microscope, by Olympus (1 mentions) Millicell ers 2 voltohmmeter, by Merck Group (1 mentions) Transwell insert, by Corning (1 mentions) Fluorescence mounting medium, by Agilent Technologies (1 mentions) Vectastain abc kit, by Vector Laboratories (1 mentions) Basement membrane matrix, by BD (1 mentions) Biozero bz 8000, by Keyence (1 mentions) Normal goat serum (ngs), by Thermo Fisher Scientific (1 mentions) Osteopontin, by Rockland Immunochemicals (1 mentions) Vinculin, by Merck Group (1 mentions) Alexa fluor 546 conjugated goat anti mouse igg, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Biozero BZ-9000 microscope Biozero BZ-8000 microscope Biozero 8000 microscope system Biozero 8000 Biozero BZ-8100 Biozero digital microscope Biozero 8100 confocal microscope Biozero BZ-8000 fluorescence microscope Biozero BZ-X700 microscope Biozero BZ-700

35 protocols using biozero

1

Localization of SLC18B1 Protein in Rat and Mouse Brains

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Indirect immunofluorescence microscopy was performed as described57 . Brain samples were obtained from male Wistar rats or C57BL/6 mice perfused intracardially with 4% (w/v) paraformaldehyde in 0.1 M phosphate buffer (pH 7.4). Cultured cells on poly-l-lysine-coated coverslips were fixed with 4% paraformaldehyde in PBS for 30 minutes. The primary antibody against mouse SLC18B1 protein was diluted 1:200 (v/v) with PBS containing 0.5% (w/v) BSA and the sample was incubated for 1 hour at room temperature. Washing steps and secondary antibody treatment were performed as described57 . The specimens were observed either under an Olympus FV300 confocal laser microscope (Olympus) or BIOZERO (Keyence).
For immunoperoxidase labeling, coronal sections of the mouse brain 30 μm thick were cut on a freezing microtome. Immunoperoxidase staining was performed on free-floating sections according to standard immunoperoxidase protocols as described58 (link). The sections were photographed with BIOZERO (Keyence) and merged to obtain whole brain images using image-joint software BZ-Analyzer (Keyence).
Hiasa M., Miyaji T., Haruna Y., Takeuchi T., Harada Y., Moriyama S., Yamamoto A., Omote H, & Moriyama Y. (2014). Identification of a mammalian vesicular polyamine transporter. Scientific Reports, 4, 6836.
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2

Fluorescence Microscopy of GFP2 Expression

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PE transgene- and BRET reporter GFP2 expression was verified with fluorescence microscopy (Keyence BioZero, Neu-Isenburg, Germany) with constant exposure times of 0.8 s.
Wimmer T., Sawinski H., Urban A.M., Motlik J, & Stieger K. (2023). Rapid and Reliable Quantification of Prime Editing Targeting Within the Porcine ABCA4 Gene Using a BRET-Based Sensor. Nucleic Acid Therapeutics, 33(3), 226-232.
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3

Quantifying PDL Cell Expression

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The middle one-third buccal aspect of the PDL on the distal palatal root was photographed using an optical microscope (Biozero; Keyence). Quantitative images were measured using image analysis software (BZ analyzer; Keyence). The number of bFGF- and VEGF-positive PDL cells was counted in a rectangular area (300×400 µm) (Fig. 2B). Three representative sections from each of the five samples of all groups were measured in a blinded manner.
Hayashi H., Terao A., Kunimatsu R, & Kawata T. (2014). Effects of a Low Level Laser on Periodontal Tissue in Hypofunctional Teeth. PLoS ONE, 9(6), e100066.
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4

Quantifying Influenza A Virus NP Localization

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A549 cells infected with FPV (MOI 5) were fixed with 4% formaldehyde for 15 min at 4°C at the indicated time points. After washing, permeabilization was performed with 0.1% TritonX-100 for 15 min at RT. Nucleoprotein (NP) localization was detected using anti-influenza A virus NP primary antibodies (AbD Serotec, #MCA-400) and Alexa Fluor® 488 chicken anti-mouse IgG secondary antibodies (Thermo Fisher Scientific, #A21200). Nuclei were counterstained with DAPI (Thermo Fisher Scientific). For quantification, two random areas were analyzed using the fluorescence microscope BIOZERO (Keyence). Quantification of nuclear localization was performed using FIJI Software's Cell Counter plugin (Schindelin et al., 2012 (link)).
Holzberg M., Boergeling Y., Schräder T., Ludwig S, & Ehrhardt C. (2017). Vemurafenib Limits Influenza A Virus Propagation by Targeting Multiple Signaling Pathways. Frontiers in Microbiology, 8, 2426.
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5

Histological Analysis of Cultured Femurs

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The cultured femurs were fixed in 10% neutral buffered formaldehyde and decalcified by immersion in 10% formic acid for 7 days. The tissue specimens were dehydrated using a graded ethanol series (50% → 70% → 100%) and embedded in paraffin. Additionally, 5 μm thick sections were obtained from paraffin-embedded specimens using a microtome. The sections were subjected to deparaffinization by immersion in a lemozole and ethanol series (100% → 90% → 70%) and then stained with Alcian blue and hematoxylin/eosin (HE). The stained sections were observed under a bright field using a fluorescence microscope (Biozero, Keyence, Osaka, Japan).
Watanabe R., Matsugaki A., Ishimoto T., Ozasa R., Matsumoto T, & Nakano T. (2022). A Novel Ex Vivo Bone Culture Model for Regulation of Collagen/Apatite Preferential Orientation by Mechanical Loading. International Journal of Molecular Sciences, 23(13), 7423.
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6

Tumor Angiogenesis and T Cell Infiltration

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Tumors were sliced into 7 μm-thick sections, fixed with 4% paraformaldehyde/PBS for 10 min at room temperature (RT), and blocked with TNB buffer [0.1 M Tris-HCl, pH 7.5, 0.15 M NaCl, 0.5% TSA blocking reagent (PerkinElmer)] for 1 hr at RT. To analyze tumor angiogenesis, sections were incubated with anti-PECAM1 (1:200 dilution; Biolegend) and -α-SMA (1:200 dilution; Abcam ab5649) antibodies at 4 °C overnight. To examine tumor-infiltrated T cells, sections were incubated with primary antibodies against CD4 (clone GK1.5) and CD8 (clone 53–6.7) (1:200 dilution; Biolegend) at 4 °C overnight, and subsequently with Alexa 488-conjugated secondary antibodies (1:1000 dilution; Invitrogen). Fluorescence signals were observed using the fluorescence microscope Biozero (KEYENCE).
Ngo Thai Bich V., Hongu T., Miura Y., Katagiri N., Ohbayashi N., Yamashita-Kanemaru Y., Shibuya A., Funakoshi Y, & Kanaho Y. (2018). Physiological function of phospholipase D2 in anti-tumor immunity: regulation of CD8+ T lymphocyte proliferation. Scientific Reports, 8, 6283.
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7

Osteoporosis in Ovariectomized Rat Model

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A postmenopausal rat model of osteoporosis was established by performing ovariectomies (OVX) of female Sprague Dawley rats [10 (link),26 (link)]. Four weeks after OVX, ALN-loaded HA gel sheets were applied to the shaved abdomens of the OVX rats for a period of 24 h every 2 weeks at a dose of 6.0 mg/rat (three pieces, 3.14 cm2 piece of gel sheet containing 2.0 mg of ALN). Separate OVX rats were subcutaneously injected with 1 mg/kg ALN every 2 weeks. Eight weeks after OVX, the rats were euthanized, and their tibias were excised, fixed using 4% paraformaldehyde in PBS, embedded in paraffin, and sectioned by microtome. The tibial sections were stained with hematoxylin and eosin and observed by microscopy (BIOZERO®; Keyence, Osaka, Japan).
Naito C., Katsumi H., Yoneto K., Omura M., Nishidono M., Kamei S., Mizoguchi A., Tamba A., Tanaka A., Morishita M, & Yamamoto A. (2019). Development of a Phosphoric Acid-Mediated Hyaluronic Acid Gel Sheet for Efficient Transdermal Delivery of Alendronate for Anti-Osteoporotic Therapy. Pharmaceutics, 11(12), 643.
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8

Quantifying Cell Orientation on Collagen

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The orientation of the cells on the substrates was examined relative to the collagen molecular orientation by taking photographs of the fluorescent phalloidin staining images of the cells using a fluorescence microscope (Biozero, Keyence, Osaka, Japan). Cell orientation was quantitatively analyzed using the Cell Profiler software (Broad Institute, Cambridge, MA) (Fig. S4). The degree of cell orientation was characterized by the orientation order parameter for the 2-dimensional system, f2D, using the following equations41 :


The degree of cell alignment f2D takes a value ranging from −1 (cells perfectly aligned perpendicular to the collagen orientation), 0 (cells oriented randomly), to 1 (cells perfectly aligned parallel to the collagen orientation).
Kimura Y., Matsugaki A., Sekita A, & Nakano T. (2017). Alteration of osteoblast arrangement via direct attack by cancer cells: New insights into bone metastasis. Scientific Reports, 7, 44824.
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9

Nrf2 Activation Evaluation in AML12 Cells

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Activation of Nrf2 in AML12 cells was evaluated by immunofluorescence microscopic observation. Cells cultured on a glass bottom dish were stimulated by GW4064 and then fixed with ice-chilled methanol for 5 min and permeabilized with 0.1% Triton X-100 in PBS for 10 min at room temperature. After blocking treatment, cells were labeled with anti-Nrf2 as a primary antibody for 1 h at room temperature, followed by incubation with a secondary antibody conjugated Alexa Fluor 488 (Thermo Fisher Scientific Inc., Waltham, MA, USA). Then nuclei were counterstained with Hoechst 33342. We investigated the expression and localization of Nrf2 in AML12 cells with a fluorescent microscope (Biozero; Keyence Corp., Osaka, Japan).
Haga S., Y.i.m.i.n, & Ozaki M. (2017). Relevance of FXR-p62/SQSTM1 pathway for survival and protection of mouse hepatocytes and liver, especially with steatosis. BMC Gastroenterology, 17, 9.
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10

Evaluating Mucosal Integrity using Caco-2 Cells

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TER and ZO-1 protein immunofluorescence. The differentiated-Caco-2 cell monolayers grown on Transwell inserts (Corning ® Inc., USA) was used to evaluate the effect of LrSKG34 on mucosal integrity in vitro, as previously described [13] . The differentiation of Caco-2 cell lines was shown as transepithelial resistance (TER) (Millicell ERS2 voltohmmeter (Merck, Millipore, USA)). The TER measurement of differentiated Caco-2 monolayers was performed based on the manufacturer's instructions (Merck Millipore). All TER measurements were conducted in triplicate. Formation of tight junctions was also evaluated as one of indicators of the differentiation of Caco-2 cell monolayers. ZO-1 protein, one of tight junction proteins, was detected using immunofluorescence in this study. Immunofluorescence assays were conducted as described in a previous study [13, 15] . For disrupting the tight junction, hydrogen peroxide in DMEM cell culture media without FBS was used in this study. Fluorescence was evaluated using fluorescence microscope (Biozero, Keyence, Japan). More than 10 images (30 ×) were taken for each condition in more than three experiments.
Sujaya I.N., Suwardana G.N.R., Gotoh K., Sumardika I.W., Nocianitri K.A., Sriwidyani N.P., Putra I.W.G.A.E., Sakaguchi M., & Fatmawati N.N.D. (2022). Potential Probiotic Characteristics and Safety Assessment of Lactobacillus rhamnosus SKG34 Isolated from Sumbawa Mare’s Milk. Microbiology and Biotechnology Letters, 50(1), 51-62.
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