Human gastric cancer BGC823 and SGC7901 cells were purchased from ATCC (Manassas, VA, USA) and cultured in RPMI-1640 (Gibco, Life Technologies, NY, USA) supplemented with 10% fetal bovine serum (FBS, ExCell Bio, Shanghai, China), 100 U/ml penicillin and 100 µg/mL streptomycin (Gibco). Plasmid DNA transfection was performed with Turbofect reagent (Invitrogen, CA, USA) according to the manufacturer’s instructions. 5-FU was purchased from Sigma and was added to subconfluent cells at the indicated doses.
Bgc 823
Manufactured by American Type Culture Collection
Sourced in United States, China
The BGC-823 is a cell line derived from human gastric adenocarcinoma. It is a commonly used in vitro model for the study of gastric cancer.
Automatically generated - may contain errors
Lab products found in correlation
Sgc 7901, by American Type Culture Collection (142 mentions)
Ges 1, by American Type Culture Collection (100 mentions)
Mgc 803, by American Type Culture Collection (94 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (94 mentions)
Mkn45, by American Type Culture Collection (82 mentions)
Hgc 27, by American Type Culture Collection (69 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (42 mentions)
Mkn 28, by American Type Culture Collection (40 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (37 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (20 mentions)
Penicillin, by Thermo Fisher Scientific (19 mentions)
Rpmi 1640, by Thermo Fisher Scientific (19 mentions)
Streptomycin, by Thermo Fisher Scientific (16 mentions)
Nci n87, by American Type Culture Collection (16 mentions)
Kato 3, by American Type Culture Collection (14 mentions)
Bgc 823, by Thermo Fisher Scientific (9 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (9 mentions)
Hek293t, by American Type Culture Collection (8 mentions)
Snu 1, by American Type Culture Collection (7 mentions)
Sgc 7901, by Thermo Fisher Scientific (7 mentions)
208 protocols using bgc 823
1
Gastric Cancer Cell Culture and Transfection
2
Gastric Cancer Cell Response to Deoxycholic Acid
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The human normal gastric epithelial cell line GES-1 and the human gastric cancer cell lines AGS, AZ-521, BGC823, SGC7901 and MKN45 (purchased from ATCC Manassas, US) were cultured in RPMI-1640 medium (Gibco, CA, US) supplemented with 10% fetal bovine serum (Biological Industries, Beit Haemek, Israel), 100 mg/ml streptomycin and 100 U/ml penicillin. All cells were incubated at 37 °C in a humidified incubator containing 5% CO2. Deoxycholic acid (DCA) (MedChem Express, US) was dissolved in dimethyl sulfoxide (DMSO). After 24 h of starvation, the cells were exposed to 50–200 µM DCA for 24 h. These doses of DCA were chosen from previous in vivo and in vitro studies [24 (link), 25 (link)].
3
Culturing Gastric Cancer Cell Lines
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Human gastric cancer cell lines including AGS, BGC-823, SGC-7901, and MGC-803, and normal human gastric mucosa epithelial cell line GES-1 were purchased from ATCC, USA. Cells were cultured in DMEM (Thermo Fisher, USA) supplemented with 10% fetal bovine serum (FBS, Thermo Fisher) in a 37°C humidified atmosphere of 5% CO2.
4
Gastric Cell Line Culturing Protocol
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The human GC cell lines SGC-7901, MKN-28 and BGC-823 (ATCC, Manassas, VA, USA) and the human normal gastric epithelial cell line GES-1 (Shanghai Institute for Life Science, Chinese Academy of Sciences, Shanghai, China) were cultured in RPMI 1640 (Solarbio, Beijing, China) supplemented with 10% fetal bovine serum (Biological industries, USA) and incubated at 37 °C, 5% CO2, and saturated humidity.
5
Gastric Cell Lines Culture Protocol
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We applied the normal human stomach epithelial cell line GES-1 (CBTCCCAS, Shanghai, China) and the human gastric adenocarcinoma cell lines AGS, SGC7901, BGC803, and BGC823 (ATCC, Manassas, VA, USA) in this study, and these cell lines were cultured in DMEM and RPMI-1640 (Invitrogen, Carlsbad, CA, USA), respectively at 37 °C in a humidified atmosphere with 5% CO2, which was supplemented with 10% fetal bovine serum (Hyclone, Logan, UT, USA), penicillin/streptomycin (1:100 dilution; Invitrogen), and 4 mM glutamine (Life Technologies, Gibco BRL).
6
Cell Lines for Gastric Cancer Research
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GES-1 is a SV40 transformed human fetal gastric epithelial cell line, which were established in 1994 and proved to maintain a normal cytoskeleton, positive in PAS reaction and were non-tumourigenic in nude mice.30 (link) GES-1, SGC7901, MKN28, MKN45 and AGS cells (originally purchased from ATCC) were maintained in RPMI-1640 medium; BGC823 and HEK293T cells (originally purchased from ATCC) were cultured in Dulbecco’s modified Eagle’s medium (Thermo Scientific HyClone, Beijing, China).
7
Gastric Cancer Cell Culture Protocol
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The GC lines MKN74, SGC7901, BGC823, MGC803, HGC27, and AGS and normal human gastric epithelial cell line GES1 were purchased from ATCC. SGC7901, BGC823, MGC803, HGC27, AGS, and GES1 were cultured in DMEM containing 10% FBS (HyClone Laboratories), 100 U/mL penicillin (Amresco), and 100 mg/mL streptomycin (Amresco). MKN74 cells were cultured in RPMI‐1640 (HyClone Laboratories) supplemented with 10% FBS, 100 U/mL penicillin, and 100 mg/mL streptomycin. All cells were incubated in a humidified atmosphere with CO2 at 37°C.
8
Culturing Human Gastric Cell Lines
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The normal human gastric mucosa cells (GES-1) and human GC cells (BGC-803, AGS, HGC-27, SGC-7901, and BGC-823) were obtained from ATCC (Shanghai, China) and cultured in McCoy’s 5a Medium (Gibco, Grand Island, NY, USA), supplemented with 10% fetal bovine serum (Gibco, sourced from Australia) and 1% streptomycin/penicillin, at a temperature of 37°C with 5% CO2.
9
Establishing 5-Fu Resistant Gastric Cancer Cell Line
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Human gastric cancer cells BGC823 were purchased from ATCC. All cells were cultured at 37 °C in a humidified 5% CO2 incubator. BGC823/5-Fu cells were established by a long term continuous exposure of BGC823 cells to 5-Fu in stepwise increase of concentration. The drug RI of BGC823/5-Fu was 31 determined by CCK8 assay.
10
Maintenance and Modification of Cell Lines
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HEK-293 T cells were maintained in Dulbecco’s modified Eagle medium (Gibco, Grand Island, NY, USA). K562 (human myelogenous leukemia cell line), AGS (human gastric adenocarcinoma), BGC-823 (human gastric adenocarcinoma), KATO III (human gastric carcinoma), and MKN-28 (human gastric carcinoma) cell lines were obtained from ATCC (Manassas, VA, USA) and maintained in RPMI-1640. Luciferase/GFP-expressing cell lines (K562-GL, AGS-GL, BGC-823-GL, KATO III-GL, MKN-28-GL) were generated by transfection of the parental cell line with lentiviral supernatant containing luciferase-2A-GFP and were sorted for GFP expression on a FACS AriaTM cell sorter (BD Biosciences, San Jose, CA, USA). DMEM and RPMI-1640 media were supplemented with 10% heat-inactivated FBS (Gibco/Life Technologies), 10 mM HEPES, 2 mM glutamine (Gibco/Life Technologies), and 1% penicillin/streptomycin. All cells were cultured at 37 °C in an atmosphere of 5% carbon dioxide.
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