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Fitc conjugated mouse specific secondary antibody

Manufactured by BioLegend
Sourced in United Kingdom, United States

FITC-conjugated mouse-specific secondary antibody is a fluorescent-labeled antibody designed to detect and bind to mouse primary antibodies. The FITC (Fluorescein Isothiocyanate) conjugate allows for the visualization and detection of target proteins or cells in various immunoassays and imaging applications.

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2 protocols using fitc conjugated mouse specific secondary antibody

1

Immunofluorescence Analysis of STAT6, CD11b, CD68, and CD163

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The following antibodies were used in the current study: STAT6 antibody, CD11b antibody, CD68 antibody, and CD163 antibody (all from Abcam, Cambridge, MA, USA). Tissue samples from STAT6−/− or WT mice were fixed with 4% paraformaldehyde for 12 h followed by 30% sucrose overnight. Texas Red-conjugated rabbit-specific secondary antibody and/or FITC-conjugated mouse-specific secondary antibody (Biolegend, London, UK) were used for immunofluorescence analysis. CD68 and CD163 fluorescence was observed, and images were captured with a Leica TCS SP8 system (Leica, Allendale, NJ, USA).
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2

Immunofluorescence Analysis of NLRP3 and CD11b

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The following antibodies were used in current studies: CD11b antibody (abcam, Cambridge, USA); NLRP3 antibody (abcam, Cambridge, USA); Tissue samples from SOD2−/+/WT mice were fixed with 4% paraformaldehyde for 12 h followed by 30% sucrose overnight. Texas Red -conjugated rabbit-specific secondary antibody and FITC-conjugated mouse-specific secondary antibody (Biolegend, USA) were used for immunofluorescence analysis of NLRP3 and CD11b respectively. NLRP3 and CD11b fluorescence expression were observed and captured by Leica TCS SP8 (Germany).
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