1h nmr 400 mhz

All the chemicals used in the present studies were of synthetic grade and were obtained from Merck specialties Ltd. and HiMedia laboratories Pvt. Ltd, (Mumbai, India). The prepared products were analysed by FT/IR (JASCO FT/IR 4100 Spectrophotometer) using KBr pellets. An LC-MS column C₆ (150 mm × 4.6 mm) with a 5 μm particle size (Shimadzu-Mass spectrophotometer) was used. The ¹H NMR spectra were recorded on a Bruker ¹H NMR 400 MHz using tetramethylsilane as an internal standard, and the chemical shifts were reported in δ ppm. The UV (Jasco V-630 Spectrophotometer) and elemental analyses for C, H, N and S were performed on a Perkin Elmer model 2400 CHNS/O analyser. A Shimadzu XRD 7000 was used for the study of the structural environment of the synthesized ligands and their metal complexes. The Faraday balance technique was employed for the measurement of magnetic susceptibility of the metal complexes. The in vitro antimicrobial activities against different bacterial and fungal pathogens were determined by the agar well diffusion method, sourced from IMTECH, India, Chandigarh.

All the chemicals (Merck specialties Ltd., Mumbai, India) used were of synthesis grade. Melting points were determined by the open capillary method (Elico), and were uncorrected. An FT/IR spectrophotometer was used to record the IR spectra of the synthesized molecules (JASCO FT/IR 4100, JASCO, JAPAN). A mass spectrophotometer with a C₆ column (150 mm × 4.6 mm, 5 μm) was used to determine the molecular mass (Shimadzu). ¹H NMR spectra were recorded using tetramethylsilane as an internal standard (Bruker ¹H NMR, 400 MHz), and chemical shifts (δ) were reported in ppm. UV and elemental analyses were performed using a JASCO V-630 spectrophotometer and a Perkin Elmer 2400 CHNS/O analyser, respectively. XRD analysis was performed using a Cu Kα X-ray source; step = 0.02(2θ), run 2θ = 2–80°, scanning speed = 2°/min (Shimadzu XRD 7000). Structure elucidation was performed using the Origin data analysis software. A scanning electron microscope (EGOMA 15 ZEISS) was used to study the structural environment. Magnetic susceptibility was analysed by a Faraday balance.
The synthesis of 5-[(4-bromo-3-methylphenyl)diazenyl]-2-hydroxybenzaldehyde (4e) was carried out as previously reported.⁶