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Pcdh ef1 cre

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The PCDH-EF1-Cre is a plasmid that contains the Cre recombinase gene under the control of the mouse protocadherin (PCDH) promoter and the EF1 enhancer. This plasmid is designed for the tissue-specific expression of Cre recombinase.

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Lab products found in correlation

Fetal bovine serum (fbs), by Harvard Bioscience (1 mentions) Crl 5800, by American Type Culture Collection (1 mentions) Puromycin, by Merck Group (1 mentions) Six well plate, by Corning (1 mentions)

2 protocols using pcdh ef1 cre

1

Lentiviral Overexpression of ORFs in NSCLC

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The ORFs of Lox and DNp63a were amplified from mouse tumour cDNAs, and ligated into expression vectors pCDH-EF1-Puro (Systems Biosciences) or pCDH-EF1-Cre (adapted vector for Lox expression in vivo). The production of lentivirus supernatant was described previously35 (link)41 (link). The human NSCLC cell lines A549 and CRL5800 (ATCC) were maintained in RPMI1640 (Hyclone) supplemented with 10% FBS (Biochrom). For transient overexpression, trypsinized cells were first incubated with virus supernatant (1:1 in volume) in a six-well plate (Corning) for 24 h, then cultured in fresh medium for another 24 h, and harvested for analysis. For stable overexpression of DNp63a, Kras/p53 ADC cells infected with virus were persistently cultured in medium with puromycin (2 μg ml−1, Sigma) until lung seeding assays.
Han X., Li F., Fang Z., Gao Y., Li F., Fang R., Yao S., Sun Y., Li L., Zhang W., Ma H., Xiao Q., Ge G., Fang J., Wang H., Zhang L., Wong K.K., Chen H., Hou Y, & Ji H. (2014). Transdifferentiation of lung adenocarcinoma in mice with Lkb1 deficiency to squamous cell carcinoma. Nature Communications, 5, 3261.
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2

Lentiviral expression of Nkx2-1 and β-catenin

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The ORFs of Nkx2-1 and N-terminal truncated β-catenin were amplified from mouse tumor cDNAs, and ligated into expression vectors pCDH-EF1-Puro (Systems Biosciences) or pCDH-EF1-Cre (adapted vector for Nkx2-1 expression in vivo). The production of lentivirus supernatant and cell infection were described previously.27 (link)
Fang Z., Han X., Chen Y., Tong X., Xue Y., Yao S., Tang S., Pan Y., Sun Y., Wang X., Jin Y., Chen H., Hu L., Hui L., Li L., Chen L, & Ji H. (2023). Oxidative stress-triggered Wnt signaling perturbation characterizes the tipping point of lung adeno-to-squamous transdifferentiation. Signal Transduction and Targeted Therapy, 8, 16.
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