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Alpha casein

Manufactured by Merck Group
Sourced in United States

Alpha casein is a type of milk protein that can be used as a laboratory reagent. It is a major component of the casein fraction of milk proteins. Alpha casein is commonly used in various biochemical and cell culture applications, but a detailed description of its specific functions is not available without the risk of unintended interpretation or extrapolation.

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2 protocols using alpha casein

1

Proteomic Sample Preparation Reagents

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Bovine pancreas TPCK-treated trypsin, bovine serum albumin (BSA), ubiquitin, cytochrome c, myoglobin, carbonic anhydrase, superoxide dismutase, alpha casein, ammonium bicarbonate (NH4HCO3), dithiothreitol (DTT), iodoacetamide (IAA), and amino acids (Gly, Pro, Cys, Ile, Leu, Asp, Lys, Glu, His, Phe, and Arg) were purchased from Sigma-Aldrich (St. Louis, MO USA). Acetonitrile (ACN), acetic acid, formic acid (FA), and hydrofluoric acid (HF) were purchased from Fisher Scientific (Pittsburgh, PA USA). Methanol was purchased from Honeywell Burdick and Jackson (Wicklow, Ireland). Water was deionized by a NanoPure system from Fisher Scientific. Complete mini protease inhibitor cocktail (provided in EASYpacks) was purchased from Roche (Indianapolis, IN USA). Fused silica capillaries (50 μm ID/360 μm OD and 30 μm ID/360 μm OD) were purchased from Polymicro Technologies (Phoenix, AZ).
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2

Purification of Membrane Proteins and Lipids

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Potassium chloride, sodium chloride, urea, imidazole, N,N-Dimethyldodecylamine N-oxide (LDAO), n-Dodecyl β-D-maltoside (DDM), and lysozyme were received from Carl-Roth. 2-Amino-2-(hydroxymethyl)−1,3-propanediol (Tris) was obtained from Roche; citric acid, bovine serum albumin (BSA), and n-hexadecane were purchased from Acros. Pentane, magnesium chloride, dithiothreitol, iodoacetamide, trypsin, beta-casein, cytochrome C, alpha-casein, sheep blood and peptides were purchased from Sigma-Aldrich (Merck), ethanol from (Boomlab). 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC), and sphingomyelin were received from Avanti Polar Lipids. Ni-NTA beads were obtained from Qiagen.
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