Talos f200 transmission electron microscope

To prepare the specimens for TEM imaging, enriched cellular constituents of BALF were primarily fixed using 2.5% glutaraldehyde + 2.0% paraformaldehyde in 0.1 M phosphate buffer (PB). The fixed cellular pellets were washed with 0.1 M PB (pH 7.4) and post-fixed with 1% osmium tetroxide in 0.1 M PB (pH 7.4) for one hour (secondary fixation) at 4 °C. For two hours, pellets were washed with distilled water, and en bloc staining was done with 2% uranyl acetate in 50% ethanol. These samples were again washed with distilled water and dehydrated in an ethanol series (50%, 70%, 80%, 90%, and 100%). These pellets were infiltrated with toluene/resin and finally embedded in Araldite CY212 resin. The blocks were polymerized at 65 °C for 48 h. Resin blocks were trimmed, and 70 nm thin sections were prepared using UC7 ultramicrotome (Leica). The sections were mounted on grids and stained with 5% uranyl acetate and 5% lead citrate. Cells were imaged using Talos F200 Transmission Electron Microscope (Thermo Fisher Scientific) using a FEG filament operated at 200 kV.

The crystal phase structure of the catalyst was characterized by a D8-Advance X-ray
diffractometer produced by Bruker, Germany. The specific surface area and mesoporous
structure of the catalyst were tested using the Micromeritics Instrument ASAP 2460
automatic rapid specific surface and porosity analyzer produced by Micromeritics
Instrument. The temperature-programmed reduction (H₂-TPR) adopted the automated
chemisorption analyzer produced by Quantachrome Instruments (Chem Star, USA) to determine
the reduction temperature of the catalyst. Determination of basic active sites on the
catalyst surface by the CO₂-TPD (AutoChem II2920) was carried out by
Micromeritics Instrument. The microstructure and element distribution of the catalyst were
observed by the TALOS F200 transmission electron microscope produced by FEI Company in the
United States. The carbon deposit of the catalyst was measured by the DSC/TGA (SDT, Q600)
type thermogravimetric analyzer produced by TA Instruments.