Mice were injected by I.P. injection with anti‐CSF‐1R (CD115) antibody (AFS98, Bio X Cell, West Lebanon, NH) or anti‐CD11b antibody (M1/70, Bio X Cell,) at a dose of 5 mg kg−1 body weight (in 300 µL dilution buffer, in vitro pure, pH = 7.4) 24 h before glycolipid administration. For the control group, the same amount of rat IgG2b (Bio X Cell) was administered. To characterize the efficacy of monocyte/macrophage depletion in vivo, mice were I.V. injected with clodronate liposomes (from Vrije University Amsterdam) at 5 mg kg−1 body weight in 200 µL PBS 48 h before glycolipid administration. For the control group, an equal amount of control PBS‐laden liposomes was administered. All liposomes were purchased from YEASEN Inc., Shanghai, China.
M1 70
Manufactured by BioXCell
The M1/70 is a flow cytometry reagent that binds to the CD11b antigen expressed on the surface of myeloid cells, including monocytes, macrophages, and granulocytes. It can be used for the identification and enumeration of these cell populations in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Rat igg2b, by BioXCell (1 mentions)
Afs98, by BioXCell (1 mentions)
Ter119, by BioLegend (1 mentions)
Ter119, by BioXCell (1 mentions)
Mr9 4, by BioLegend (1 mentions)
Pc 61, by BioXCell (1 mentions)
Ebio927, by Thermo Fisher Scientific (1 mentions)
M1 70, by BioLegend (1 mentions)
Rb6 8c5, by BioXCell (1 mentions)
2 protocols using m1 70
1
In Vivo Macrophage Depletion Protocol
2
Characterizing Mouse Immune Cell Phenotypes
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Antibodies directed against the following mouse markers were used: CD3 (145–2C11, Tonbo Biosciences 60–0031), CD4 (RM4–5, BioLegend 100559; GK1.5, BioXCell BE0003–1), CD8 (53–6.7, Tonbo Biosciences 75–0081; 53.6.72, BioXCell BE0004), CD11b (M1/70, BioLegend 101204; M1/70, BioXCell BE0007), CD11c (N418, BioLegend 117322), CD19 (6D5, BioLegend 115534), CD25 (PC61, BioLegend 102024; PC61.5.3, BioXCell BE0012), CD45 (30-F11, BioLegend 103138), CD45.1 (A20, BioLegend 110714), CD69 (H1.2F3, BioLegend 104504), CD80 (16–10A1, BioLegend 104724), B220 (RA3–6B2, BioLegend 103232; RA3.3A1/6.1, BioXCell BE0067), AIRE (5H12, eBioscience 53–5934–82), EpCAM (G8.8, BioLegend 118206), F4/80 (BM8, BioLegend 123116), Gr-1 (RB6–8C5, BioLegend 108410; RB6–8C5, BioXCell BE0075), I-A/I-E (M5/114.15.2, BioLegend 107628), NK1.1 (PK136, BioLegend 108716), PDCA1 (eBio927, eBioScience 12–3172–82), Sirpα (P84, BioLegend 144008), TER-119 (TER-119, BioLegend 116210; TER-119, BioXCell BE0183), Vα2 (B20.1, BioLegend 127818), Vβ5 (MR9–4, BioLegend 139504), and XCR1 (ZET, BioLegend 148212). For immunostaining ~107 cells in 100 μL of PBS + 2% bovine calf serum (BCS), fluorochrome-conjugated antibodies were diluted from stock concentrations of 0.5 mg mL−1 and incubated with cells for 20 min on ice, unless specified.
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