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Anti human cd16 clone 3g8

Manufactured by BioLegend
Sourced in United States

Anti-human CD16 (clone 3G8) is a monoclonal antibody that recognizes the CD16 antigen expressed on human cells. CD16 is a low-affinity Fc receptor that binds to the Fc portion of IgG antibodies and plays a role in antibody-dependent cell-mediated cytotoxicity (ADCC).

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2 protocols using anti human cd16 clone 3g8

1

Flow Cytometry Analysis of Hematopoietic Markers

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For analysis of surface markers, cells were stained in PBS containing 2% (w/v) BSA, with the following antibodies (from Biolegend): anti-human CD34 (clone 581, Cat# 343504, 1:200), anti-human CD33 (clone P67.6, Cat# 366608, 1:200), anti-human CD117 (clone 104D2, Cat# 313205, 1:200), anti-human CD16 (clone 3G8, Cat# 302046, 1:100), anti-human CD14 (clone 63D3, Cat# 367117, 1:200). Monocyte and macrophage were indicated by anti-human CD14+. Flow cytometry data were acquired on a LSRII or LSR Fortessa (BD Biosciences) and analyzed using FlowJo software (Tree Star).
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2

Co-culture Assay for T Cell Activation

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Raji cells were co-cultured for 24 h with T cells in a 96-well flat bottom plate (Corning, Kaiserslautern, Germany) in DMEM supplemented with 0.1 mM NEAA, 1 mM sodium pyruvate (Life Technologies, Darmstadt, Germany), and different antibodies (antibody concentration 1 µg/ml) in a ratio of 10:1. Prior to surface staining, Fc-block was performed using Human TruStain FcX™ (BioLegend, USA). Subsequently, cells were stained with anti-human CD19 (clone HIB19, BioLegend, USA), anti-human CD3 (clone HIT3a, BioLegend, USA), and anti-human CD16 (clone 3G8, BioLegend, USA) in PBS. A live-dead stain (Zombie Aqua™ Fixable Viability Kit, BioLegend, USA) was used to limit analysis to viable cells. Cell numbers were determined by Count Bright absolute counting beads (Life Technologies, USA).
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