Recombinant il 13

Manufactured by R&D Systems

Sourced in United States

Recombinant IL-13 is a protein produced using recombinant DNA technology. It is a member of the interleukin family of cytokines. Interleukin-13 plays a role in immune and inflammatory responses.

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Lab products found in correlation

Recombinant il 4, by R&D Systems (2 mentions) Recombinant ifn γ, by R&D Systems (2 mentions) Ficoll paque plus, by Merck Group (1 mentions) Recombinant human m csf, by R&D Systems (1 mentions) Transwell permeable support, by Corning (1 mentions) Bz 9000, by Keyence (1 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions) L6529, by Merck Group (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Cd11b, by BD (1 mentions) F4 80, by Thermo Fisher Scientific (1 mentions) Recombinant il 10, by R&D Systems (1 mentions) Cd206, by BD (1 mentions) Antibiotic and antimycotic solution, by Merck Group (1 mentions) Dispase, by BD (1 mentions) Rat tail derived collagen 1, by Merck Group (1 mentions) Rneasy mini kit, by Qiagen (1 mentions) Fitc conjugated f4 80 antibody, by BD (1 mentions) Recombinant m csf, by R&D Systems (1 mentions) Dmem f12, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

IL-13 (210 citations) Recombinant human IL-13 (10 citations) Recombinant mouse IL-13 (4 citations) Recombinant murine IL-13 (3 citations) Recombinant human IL-4 and IL-13 (3 citations) Recombinant human IL-13 protein (2 citations)

7 protocols using recombinant il 13

Macrophage Differentiation and Co-culture

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For in vitro experiments on human peripheral blood mononuclear cell (PBMC)-derived macrophages, human peripheral blood was obtained from allergic rhinitis patients. Patient blood was diluted in PBS and centrifuged with Ficoll-Paque PLUS (Sigma-Aldrich). PBMCs were isolated and differentiated using 50 ng/ml human recombinant M-CSF (R&D Systems). All patients provided written informed consent, and the study was approved by the Seoul National University Hospital Institutional Review Committee (1610-062-799).
The murine AM cell line MH-S (ATCC, USA) was seeded under the same conditions and stimulated with 20 ng/ml recombinant IL-13 (R&D Systems), with or without hUC-MSCs. In addition, a transwell assay was performed using Transwell Permeable Support (Costar). In macrophage in vitro experiments, 1 × 10⁵ macrophages were co-cultured with 1 × 10⁴ hUC-MSCs.

Mo Y., Kang S.Y., Bang J.Y., Kim Y., Jeong J., Jeong E.M., Kim H.Y., Cho S.H, & Kang H.R. (2022). Intravenous Mesenchymal Stem Cell Administration Modulates Monocytes/Macrophages and Ameliorates Asthmatic Airway Inflammation in a Murine Asthma Model. Molecules and Cells, 45(11), 833-845.

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HREC Migration Assay using Boyden Chamber

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Migration of HRECs was determined using a modified Boyden chamber containing polycarbonate membranes (Transwell, 8-μm pore size; Corning) coated with 10 μg/mL of collagen for 1 hr at 37°C. Starved HRECs were seeded into the upper chamber at 2 × 10⁴ cells/insert in medium containing recombinant POSTN (R&D Systems) or recombinant IL-13 (50 ng/mL; R&D Systems). The lower chamber was filled with medium without serum and growth factors. After 16 hr of incubation at 37°C, non-migrated cells on the upper surface of the membrane were removed by gentle scraping with cotton swabs, and the migrated cells on the lower surface of the membrane were stained with Hoechst 33342 (Molecular Probes). Three photographs were taken of each insert at randomly selected sites with a fluorescence microscope (BZ-9000; Keyence). The number of migrated cells was counted using Adobe Photoshop CS6.

Nakama T., Yoshida S., Ishikawa K., Kubo Y., Kobayashi Y., Zhou Y., Nakao S., Hisatomi T., Ikeda Y., Takao K., Yoshikawa K., Matsuda A., Ono J., Ohta S., Izuhara K., Kudo A., Sonoda K.H, & Ishibashi T. (2017). Therapeutic Effect of Novel Single-Stranded RNAi Agent Targeting Periostin in Eyes with Retinal Neovascularization. Molecular Therapy. Nucleic Acids, 6, 279-289.

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Gremlin-1 and Collagen Regulation

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Healthy dermal fibroblasts were culture in standard six well plates until confluent. Media was removed and replaced with media containing recombinant IL-13 100 ng/ml (R&D Systems, United Kingdom) at which we have previously demonstrated to give a robust increase in collagen at both mRNA and protein levels in fibroblasts (O’Reilly et al., 2016 ). After 24 and 48 h cells were lysed and Gremlin-1 quantified by qRT-PCR. At 24 h Col1A1 was also quantified by qRT-PCR.

Duffy L., Henderson J., Brown M., Pryzborski S., Fullard N., Summa L., Distler J.H., Stratton R, & O’Reilly S. (2021). Bone Morphogenetic Protein Antagonist Gremlin-1 Increases Myofibroblast Transition in Dermal Fibroblasts: Implications for Systemic Sclerosis. Frontiers in Cell and Developmental Biology, 9, 681061.

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Polarization of Macrophage Subtypes

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Type I subset and M2 macrophages were polarized as described previously (29 (link)). Primary BMDM macrophages were polarized toward the M1 phenotype with recombinant IFN-γ (100 U/ml, R&D Systems 485-ML) and/or LPS (100 ng/ml, L6529; strain 055:B5; Sigma-Aldrich, St. Louis, MO, USA) or toward the M2 phenotype with recombinant IL-4 (R&D Systems 404-ML) or recombinant IL-13 (R&D Systems 413-ML) (10 ng/ml) for the specified times under normal culture conditions. Unpolarized cells (M0) served as controls. BMDMs were washed with PBS and harvested for total RNA isolation.

Das A., Yang C.S., Arifuzzaman S., Kim S., Kim S.Y., Jung K.H., Lee Y.S, & Chai Y.G. (2018). High-Resolution Mapping and Dynamics of the Transcriptome, Transcription Factors, and Transcription Co-Factor Networks in Classically and Alternatively Activated Macrophages. Frontiers in Immunology, 9, 22.

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Polarization of Inflammatory and Anti-Inflammatory Macrophages

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For inflammatory macrophage (M1) differentiation, MФ were stimulated with 100 ng/mL LPS and 100 ng/mL recombinant IFN‐γ (R&D Systems, Minneapolis, MN, USA) for 24 hours. To generate anti‐inflammatory macrophages (M2), MФ were stimulated with 100 ng/mL recombinant IL‐4 (R&D Systems), 20ng/mL recombinant IL‐10 (R&D Systems) and 100 ng/mL recombinant IL‐13 (R&D Systems) for 24 hours. Polarized cells were identified by flow cytometry with myeloid and lymphoid immunophenotyping panels. Primary antibodies used in the flow cytometry analysis are as follows: CD11b (BD Biosciences, Franklin Lakes, NJ, USA), CD206 (BD Biosciences) and F4/80 (Thermo Fisher Scientific, Waltham, MA, USA). Data were collected using a BD LSRFortessa analyser and analysed using FlowJo 10.0 software.

Juan C., Mao Y., Cao Q., Chen Y., Zhou L., Li S., Chen H., Chen J., Zhou G, & Jin R. (2021). Exosome‐mediated pyroptosis of miR‐93‐TXNIP‐NLRP3 leads to functional difference between M1 and M2 macrophages in sepsis‐induced acute kidney injury. Journal of Cellular and Molecular Medicine, 25(10), 4786-4799.

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Isolation and Culture of Murine Keratinocytes

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Body wall skin from neonatal mice was incubated overnight at 4 °C in 5 U ml⁻¹ Dispase (BD) solution supplemented with 1 × antibiotic and antimycotic solution (Sigma). The epidermis was isolated and further digested in TrypLE Express solution supplemented with 200 μg ml⁻¹ DNAse I and DNAse buffer. Cell suspensions were filtered, resuspended in defined KC serum-free medium with supplements (Life)+1 × antibiotic–antimycotic solution. KCs were seeded at an appropriate cell density onto tissue culture vessels coated with rat-tail-derived-collagen I (Sigma). Culture vessels were washed with PBS 24 h following seeding to remove unattached cells, and provided with fresh medium±20 ng ml⁻¹ recombinant IL-13 (R&D). For extraction of KCs, cell culture vessels were treated with trypsin and KC RNA was subsequently extracted using the RNEasy Mini kit (Qiagen).

Dalessandri T., Crawford G., Hayes M., Castro Seoane R, & Strid J. (2016). IL-13 from intraepithelial lymphocytes regulates tissue homeostasis and protects against carcinogenesis in the skin. Nature Communications, 7, 12080.

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Isolating and Polarizing Mouse Macrophages

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Mouse macrophages were isolated from bone marrow, as described before [37 (link)]. Marrow from male, 12 week-old BALB/C mice was flushed out with PBS containing 20mmol/l Tris and 100mmol/l NaCl (pH 7.5) through a 23-gauge needle. Cells were pre-treated with FITC-conjugated F4/80 antibody (Becton-Dickinson Biosciences, San Jose, CA, USA) and then sorted for F4/80+ cells by flow cytometry. Purified F4/80+ macrophages were cultured in Dulbecco’s Modified Eagle Medium/F12 (DMEM/F12; Invitrogen, St. Louis, MO, USA) suppled with 10mmol/l L-glutamine, 100U/ml penicillin, 100µg/ml streptomycin and 100U/ml recombinant M-CSF (R&D Systems). For induction of macrophage polarization in vitro by IL-13, cultured macrophages were treated with recombinant IL-13 (R&D Systems, cat. no. 413-M) at a concentration of 20 U/ml and incubate overnight.

Yang H., Chen Y, & Gao C. (2017). Interleukin-13 reduces cardiac injury and prevents heart dysfunction in viral myocarditis via enhanced M2 macrophage polarization. Oncotarget, 8(59), 99495-99503.

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