4-arm poly(ethylene glycol)-amine (20 kDa) was purchased from JenKem Technology USA. Reagents and chemicals for peptide synthesis were were acquired from Anaspec or Chempep. Bovine type I collagen was purchased from Amsbio. AlamarBlue reagents were purchased from AbD Serotec. Live/Dead staining kit for mammalian cells and DAPI stain were obtained from Invitrogen. Gemcitabine was purchased from TSZ CHEM. YAP rabbit mAb, E-cadherin rabbit mAb, vimentin rabbit mAb, anti-rabbit IgG, anti-mouse IgG HRP-linked, and Alexa Fluor® 488-labeled anti-mouse IgG F(ab’)2 antibodies were obtained from Cell Signaling Technology. hVEGF ELISA kit was purchased from PeproTech. HPLC grade acetonitrile and water were acquired from Fisher Scientific and VWR International, respectively. All other chemicals were purchased from Sigma-Aldrich unless noted otherwise.
Yap rabbit mab
Manufactured by Cell Signaling Technology
Sourced in United States
YAP rabbit mAb is a monoclonal antibody that is used to detect the protein YAP (Yes-associated protein) in various biological samples. YAP is a transcriptional co-activator that plays a critical role in the Hippo signaling pathway, which regulates cell growth, proliferation, and apoptosis.
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Lab products found in correlation
2 protocols using yap rabbit mab
1
Peptide-Hydrogel Scaffold for Cell Culture
2
VEGF-Induced YAP Localization in BMSCs
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BMSCs were cultured on 24-well plates with cover glasses. After treatment with or without VEGF for 24 h, cells were fixed with 4% paraformaldehyde for 15 min and treated with 0.2% Triton X-100. Some cells were incubated with phalloidin (Molecular Probes) solution for 30 min, and the nuclei were labelled with 4,-6-diamidino-2-phenylindole (DAPI, Invitrogen, USA). Others were blocked with 5% goat serum for 30 min and incubated with YAP rabbit mAb (Cell Signaling Technology, USA) overnight at 4 ℃. Afterwards, the cells were incubated with Alexa Fluor 488-conjugated goat anti-rabbit antibody (Bioss, China) for 90 min at room temperature. Finally, the nuclei were labelled with DAPI. The cells on the slides were visualized by a spectral confocal laser-scanning microscope (LSCM, Germany).
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