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Photometric coolsnap hq camera

Manufactured by Teledyne
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Sourced in Germany, United States

The Photometric CoolSNAP HQ camera is a scientific-grade digital camera designed for high-performance imaging applications. It features a high-resolution, back-illuminated CCD sensor and advanced cooling technology to ensure low noise and high sensitivity.

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Lab products found in correlation

Inverted eclipse te300 microscope, by Teledyne (2 mentions) Personal deltavision microscope, by Cytiva (1 mentions) Deltavision personal system, by Cytiva (1 mentions)

Close spelling variants of this product

Photometrics CoolSnap HQ camera Photometrics CoolSNAP HQ CoolSNAP HQ camera CoolSNAP HQ CoolSNAP camera

4 protocols using photometric coolsnap hq camera

1

Visualizing Actin Dynamics in Dictyostelium

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Wild-type Dictyostelium (AX2) cells expressing LimE∆-RFP were cultured at room temperature in HL5 media containing 75 mM glucose with appropriate antibiotics. Cells were washed twice with KK2 buffer and transferred to glass-bottomed imaging culture plates (Fisher Scientific UK, Loughborough, UK). Actin was visualized using a Personal DeltaVision microscope (Applied Precision, Issaquah, WA) comprising an Olympus UPlanSApo 100×, NA 1.4, oil immersion objective and a Photometric CoolSNAP HQ camera (Roper Scientific, Martinsried, Germany). Captured images were processed by iterative constrained deconvolution using SoftWoRx (Applied Precession) and analyzed using ImageJ (20 ).
Lockley R., Ladds G, & Bretschneider T. (2014). Image Based Validation of Dynamical Models for Cell Reorientation. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 87(6), 471-480.
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2

Traction Force Microscopy of Cells

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Traction of pancreatic cells was measured as previously described18 (link),66 . Images of cells on PA gels containing fluorescent beads were collected before and after trypsinization using a Nikon Inverted Eclipse TE300 microscope and a Photometric Cool Snap HQ camera (Roper Scientific). TFMs were calculated based on differences in bead displacement induced by substrate deformation and relaxation using a software analysis program.
Laklai H., Miroshnikova Y.A., Pickup M.W., Collisson E.A., Kim G.E., Barrett A.S., Hill R.C., Lakins J.N., Schlaepfer D.D., Mouw J.K., LeBleu V.S., Roy N., Novitskiy S.V., Johansen J.S., Poli V., Kalluri R., Iacobuzio-Donahue C.A., Wood L.D., Hebrok M., Hansen K., Moses H.L, & Weaver V.M. (2016). Genotype tunes pancreatic ductal adenocarcinoma tissue tension to induce matricellular-fibrosis and tumor progression. Nature medicine, 22(5), 497-505.
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3

Traction Force Microscopy of Cells

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Traction of pancreatic cells was measured as previously described18 (link),66 . Images of cells on PA gels containing fluorescent beads were collected before and after trypsinization using a Nikon Inverted Eclipse TE300 microscope and a Photometric Cool Snap HQ camera (Roper Scientific). TFMs were calculated based on differences in bead displacement induced by substrate deformation and relaxation using a software analysis program.
Laklai H., Miroshnikova Y.A., Pickup M.W., Collisson E.A., Kim G.E., Barrett A.S., Hill R.C., Lakins J.N., Schlaepfer D.D., Mouw J.K., LeBleu V.S., Roy N., Novitskiy S.V., Johansen J.S., Poli V., Kalluri R., Iacobuzio-Donahue C.A., Wood L.D., Hebrok M., Hansen K., Moses H.L, & Weaver V.M. (2016). Genotype tunes pancreatic ductal adenocarcinoma tissue tension to induce matricellular-fibrosis and tumor progression. Nature medicine, 22(5), 497-505.
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4

Visualizing GLP-1 Receptor Expression in Yeast

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To visualize GLP-1 receptor expression in yeast cells, a C-terminal in-frame fusion construct between the GLP-1 receptor and 3xmCherry ORFs fused together (separated by a Asp-Gly linker) was generated using a two-step cloning technique as described previously (Ladds et al., 2005b (link)). To generate an expression cassette, GLP-1 receptor-3xmCherry was cloned into pRS306 containing the GAPDH promoter and the CYC1 terminator sequence separated by a BamHI restriction site. This was integrated at the ura3 locus. Positive transformants were grown in YPDA to a density of 5 × 106 cells mL−1. Then 100 μL of culture was harvested and the cells washed in growth media. Cell suspension (3 μL) was transferred to slides (Sigma) and viewed using a Personal DeltaVision system (Applied Precision, Issaquah, WA, USA) equipped with a Photometric CoolSNAP HQ camera (Roper Scientific, Trenton, NJ, USA). Deconvolution was applied to images for visual clarity.
Weston C., Poyner D., Patel V., Dowell S, & Ladds G. (2014). Investigating G protein signalling bias at the glucagon-like peptide-1 receptor in yeast. British Journal of Pharmacology, 171(15), 3651-3665.
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