Anti aff4 ab57077 antibody

Manufactured by Abcam

Anti-AFF4 (ab57077) antibody is a primary antibody that recognizes the AFF4 protein. AFF4 is a transcriptional elongation factor that is part of the super elongation complex. This antibody can be used in various applications to detect and study the AFF4 protein.

Automatically generated - may contain errors

Lab products found in correlation

Ell1 a301 645a, by Fortis Life Sciences (2 mentions) Enl a302 268a, by Fortis Life Sciences (1 mentions) Ha 3f10, by Merck Group (1 mentions) Ha tag, by Addgene (1 mentions) β catenin e 5, by Santa Cruz Biotechnology (1 mentions) Flag m2, by Roche (1 mentions)

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Anti-AFF4 (2 citations)

2 protocols using anti aff4 ab57077 antibody

Antibodies for Transcription Regulation

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Polyclonal antibodies against AFF1 (A302-344A), ELL1 (A301-645A), ELL2 (A302-505A), ENL (A302-268A) and AF9 (A300-595A) were purchased from Bethyl Laboratories. Anti-AFF4 (ab57077) antibody was purchased from Abcam. The monoclonal antibodies against Flag (M2) and HA (3F10) were from Sigma-Aldrich and Roche, respectively. The antibodies against CDK9, LARP7 and HEXIM1 were generated in our own laboratory and have been described previously (17 (link),18 (link)).

Lu H., Li Z., Zhang W., Schulze-Gahmen U., Xue Y, & Zhou Q. (2015). Gene target specificity of the Super Elongation Complex (SEC) family: how HIV-1 Tat employs selected SEC members to activate viral transcription. Nucleic Acids Research, 43(12), 5868-5879.

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Immunoblotting Assays for HCF1, HCF2, and Related Factors

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The antibodies against HCF1 (sc-390950), HCF2 (sc-393250), Cyclin T1 (H-245), TRAF2 (F-2) and β-Catenin (E-5) were purchased from Santa Cruz Biotechnology. The anti-AFF4 (ab57077) antibody was purchased from Abcam. The anti-AFF1 (A302-344A), -ENL (A302-268A), -AF9 (A300-595A), -ELL2 (A302-505A) and -ELL1 (A301-645A) antibodies were purchased from Bethyl Laboratories. The monoclonal antibodies against HA (3F10) and Flag (M2) were from Roche and Sigma-Aldrich, respectively. The antibodies against CDK9, LARP7 and Brd4 were generated in our own laboratory and have been described previously (14 (link),15 (link)).
The HCF1 cDNA (pCGN-HCF-1; plasmid #53309) with an HA-Tag at the N-terminus and a Myc tag at the C-terminus was purchased from Addgene. The HCF2 cDNA (BC033799) was purchased from transOMIC technologies. The HCF2 cDNA was cloned into expression vectors N-Flag-PRK5M and N-HA-PRK5M, respectively.

Wu J., Xue Y., Gao X, & Zhou Q. (2020). Host cell factors stimulate HIV-1 transcription by antagonizing substrate-binding function of Siah1 ubiquitin ligase to stabilize transcription elongation factor ELL2. Nucleic Acids Research, 48(13), 7321-7332.

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