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Sd ldl ex seiken

Manufactured by Denka Seiken
Sourced in Japan

The Sd-LDL-EX 'SEIKEN' is a laboratory equipment used for the measurement of small dense low-density lipoprotein (sd-LDL) levels. It is designed to provide accurate and reliable results for clinical and research applications.

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2 protocols using sd ldl ex seiken

1

Comprehensive Lipid and Fatty Acid Profiling

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Blood sample were centrifuged at 3000 rpm for 10 min at 4°C and then separated into plasma or serum. Alpha-tocopherol was measured using high-performance liquid chromatography. Total cholesterol was determined by the cholesterol dehydrogenase (UV-End) method. LDL-cholesterol and HDL-cholesterol were determined by a direct method. We calculated non-HDL cholesterol as the difference between total and HDL cholesterol. TG was determined by an enzyme method. The remnant like particles (RLP) -cholesterol was determined by the immune adherence method. Cholesterol ester transfer protein activity (CETP) was measured using a commercially available ELISA kit (Daiichi-kagaku, Tokyo Japan). Apolipoproteins (apo A-1, B, C-3 and E) were measured by the turbidimetric immunoassay (TIA) system. Apo B-48 was measured by the chemiluminescent enzyme immunoassay (CLEIA). Serum fatty acids composition was measured by gas–liquid chromatography. Briefly, total lipids in the serum were extracted using the Folch procedure and fatty acids were then methylated with BF3/methanol. Transesterified fatty acids was then analyzed using a gas chromatograph (GC-17A; Shimadzu, Kyoto, Japan) with a capillary column Omegawax 250 (Supelco, Bellefonte, PA). Measurements of sd-LDL-cholesterol were performed using sd-LDL-EX “SEIKEN” (Denka Seiken, Tokyo, Japan).
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2

Genetic Associations of Lipoprotein Profiles

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Concentrations of lipoprotein particles were measured at LipoScience, Inc. (Raleigh, NC) using NMR spectroscopy on plasma EDTA specimens. LipoScience has developed validated software for analysis of NMR measured LipoProfile spectra that uses an optimized deconvolution algorithm to quantify lipoprotein subspecies66 (link),67 (link). MESA was measured with the LipoProfile-III assay while FHS samples were measured with the LipoProfile-I assay, which provides less accuracy for some measurements but is similar to LipoProtein-III. We associated lipoprotein profiles with top associated SNPs within up to 1,802 FHS and 4551 MESA participants adjusting for age, sex, and lipid-lowering therapy.
For individuals who participated in ARIC study visit 4 (1996–1998), a homogeneous assay method was used for the direct measurement of sdLDL-C in plasma (sd-LDL-EX “Seiken”, Denka Seiken, Tokyo, Japan) on a Hitachi 917 automated chemistry analyzer68 (link). We associated top associated SNPs with ARIC participants adjusting for age, sex, lipid-lowering therapy, race, study center, and the first 11 principal components of ancestry.
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