Soybean lecithin (SPC) was purchased from Shanghai Tai Wei Chemical Company (Shanghai, China). DSPE-PEG2000 was bought from Avanti Polar Lipids (Alabaster, AL, USA). DSPE-PEG-Mal (SUNBRIGHTDSPE-0.20MA) and NBD-DPPE (COATSOME FE-6060NB) was purchased from NOF Co. Ltd. (Tokyo, Japan). BR2 peptide, cys-RAGLQFPVGRLLRRLLR, was provided by SciLight Biotechnology (Beijing, China). Cantharidin (CTD) was obtained from Chengdu Biopurify Phytochemicals Ltd. (Sichuan, China). The rabbit monoclonal anti-CA IX antibody targeting the N-terminal region of the protein that is exposed to the extracellular side was obtained from Abcam (Cambridge, UK). The goat anti-rabbit horseradish peroxidase (HRP)-conjugated secondary antibody was obtained from Bio-rad Laboratories (Hercules, CA, USA). D-luciferin was purchased from Onwon Inc., Hong Kong, China. Hoechst 33342 and the fluorescent LysoTracker Red DND-99 was purchased from Molecular Probes Inc. (Eugene, OR, USA). The lipophilic near-infrared fluorescent dye 1,1′-dioctadecyltetramethyl indotricarbocyanine iodide (DiR) used for labeling liposomes was supplied by Caliper LifeSciences (Hopkinton, MA, USA). All other reagents are purchased from Sigma otherwise it was specified.
Goat anti rabbit horseradish peroxidase hrp conjugated secondary antibody
Manufactured by Bio-Rad
Sourced in United States
Goat anti-rabbit horseradish peroxidase (HRP)-conjugated secondary antibody is a reagent used in immunoassay techniques, such as Western blotting and ELISA, to detect and quantify target proteins. The secondary antibody binds to the primary antibody that is specific to the target protein, and the conjugated HRP enzyme enables colorimetric or chemiluminescent detection.
Automatically generated - may contain errors
Lab products found in correlation
Hoechst 33342, by Thermo Fisher Scientific (1 mentions)
Lysotracker red dnd 99, by Thermo Fisher Scientific (1 mentions)
Dspe peg2000, by Avanti Polar Lipids (1 mentions)
Cantharidin ctd, by Chengdu Biopurify Phytochemicals (1 mentions)
Chemidoc mp imaging system, by Bio-Rad (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Millipore water purification system, by Merck Group (1 mentions)
Dithiotreitol dtt, by Merck Group (1 mentions)
Laemmli buffer, by Carl Roth (1 mentions)
Complete protease inhibitor cocktail, by Roche (1 mentions)
Rabbit anti tfam, by Abcam (1 mentions)
Rabbit anti pgc 1α, by Abcam (1 mentions)
Antibody against gapdh, by Abcam (1 mentions)
Nitrocellulose, by Bio-Rad (1 mentions)
5 protocols using goat anti rabbit horseradish peroxidase hrp conjugated secondary antibody
1
Preparation and Characterization of Theranostic Liposomes
2
SDS-PAGE Analysis of Reduced and Non-Reduced RBD
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The samples incubated as described above were diluted in non‐reducing Sodium Dodecyl Sulphate (SDS) sample buffer, heated at 100°C, and resolved by SDS‐PAGE. Fully reduced [R] and non‐reduced [NR] RBD used as a reference were obtained by diluting the protein in SDS sample buffer containing or not 4% (v/v) β‐mercaptoethanol, respectively. For detection, the protein was blotted onto PVDF membranes and immune‐stained with anti 6X His tag rabbit polyclonal antibody (OriGene Technologies Inc, Rockville, MD, USA). Detection was performed using a goat anti‐rabbit horseradish peroxidase (HRP)‐conjugated secondary antibody (Bio‐Rad, Hercules, CA, USA) and the enhanced chemiluminescence detection kit WesternBright ECL (Advasta, San Jose, CA, USA). Immunoreactive bands were visualized in a ChemiDoc MP Imaging System (Bio‐Rad).
3
Antibody-Targeted Liposomal Drug Delivery
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Soybean phosphatidylcholine (SPC) was purchased from Taiwei Pharmaceutical Co, Ltd (Shanghai, People’s Republic of China). N-[(3-maleimide-1-oxopropyl)aminopropyl polyethyleneglycol-carbamyl] distearoylphosphatidyl-ethanolamine (DSPE-PEG-MAL) was purchased from NOF America Corporation (White Plains, NY, USA). DTX was supplied by LC Laboratories (Woburn, MA, USA). The rabbit monoclonal anti-CA IX antibody targeting the N-terminal region of the antigen protein that is exposed to the extracellular side was obtained from Abcam (Cambridge, UK). The goat antirabbit horseradish peroxidase (HRP)-conjugated secondary antibody was obtained from Bio-rad Laboratories (Hercules, CA, USA). Dithiotreitol (DTT) was purchased from Sigma-Aldrich (St Louis, MO, USA). The lipophilic near-infrared fluorescent dye 1,1′-dioctadecyltetramethyl indotricarbocyanine iodide (DiR) used for labeling liposomes was supplied by Caliper LifeSciences (Hopkinton, MA, USA). Acetonitrile of HPLC grade and ethyl acetate were obtained from Anaqua Chemicals Supply (Houston, TX, USA). Dimethyl sulfoxide (DMSO) was purchased from Sigma-Aldrich. Ultrapure water was generated by a Millipore water purification system (EMD Millipore, Billerica, MA, USA).
4
Immunoblotting of T Cell Metabolism
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Cell lysates were prepared in RIPA buffer supplemented with a complete protease-inhibitor cocktail (Roche) and mixed in equal amounts with Laemmli buffer (Roth) before denaturation at 98 °C for 5 min. Protein quantification was performed with the Pierce 660 nm Protein Assay (Pierce). Protein samples were separated via SDS-PAGE, blotted onto nitrocellulose membranes, blocked with 5% BSA, and incubated with monoclonal rabbit anti-HIF-1α (Cell Signaling Technology, clone D1S7W). Detection was carried out with a goat anti-rabbit horse radish peroxidase (HRP) conjugated secondary antibody (BioRad), visualized with the enhanced chemiluminescent SuperSignal reagent (Pierce). For the detection of PGC-1α, TFAM, and elector transport chain (ETC) complex expression, Tpex and Tex cells were FACS sorted from LCMVCL13 infected mice 30 d.p.i. and 5x105 T cells were directly lysed in RIPA buffer. Detection of PGC-1α, TFAM, and ETC complexes was carried out using rabbit anti-PGC-1α (Abcam), rabbit-anti-TFAM (Abcam), and an OxPhos rodent antibody cocktail (Thermo) with goat anti-mouse or rabbit HRP-conjugated secondary antibodies (both BioRad).
5
Western Blot Analysis of USP47
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Total protein lysates were fractionated on 4–15% polyacrylamide gels and transferred onto nitrocellulose (Bio-Rad, Richmond, CA, USA). The levels of USP47 were analyzed by western blots using a rabbit anti-USP47 (Thermo Fisher Scientific) with dilution of 1:500. Normalization was performed by blotting the same samples with an antibody against GAPDH (Abcam, Cambridge, MA, USA). The secondary antibodies were goat anti-rabbit horseradish peroxidase (HRP)-conjugated secondary antibody (1:2,000; Bio-Rad).
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