Total RNA extraction was performed with TRIzol Reagent (Life, 139505) and cDNA was synthesized using a cDNA reverse transcription kit (Roache). Amplification and detection were performed on a Light Cycler 480 Sybr System (Roche Applied Science) with the SYBR Green qPCR SuperMix (Promega) For quantitative RT-PCR, β-actin was used as an endogenous control. The primers are shown in Supplementary Table 1 .
Light cycler 480 sybr system
Manufactured by Roche
The Roche Light Cycler 480 Sybr System is a real-time PCR (polymerase chain reaction) instrument designed for quantitative gene expression analysis. It utilizes SYBR Green I as the detection dye, allowing for the measurement of target gene amplification in real-time. The system offers precise temperature control, high-throughput capabilities, and user-friendly software for data analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using light cycler 480 sybr system
1
Quantitative RT-PCR for Gene Expression
2
Quantification of Hepatic Gene Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA isolated from liver samples was used. The cDNA synthesis of total RNA was carried out using a ReverTra Ace qPCR RT kit (TOYOBO, Osaka, Japan) according to the manufacturer’s instructions. To measure the mRNA amount in liver samples, RT-PCR was conducted with SYBR green dye using the LightCycler 480 SYBR system (Roche Applied Science, Mannheim, Germany). The oligonucleotide primers used for amplification were obtained from commercial products (Adh1, Aldh1, Aldh2, Gsta2, Gsta4, Gstm1, Gstt2, Sod1 and Actb (β-actin) (Takara Bio, Shiga, Japan)). The expression of each gene was normalized to that of β-actin mRNA.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!