For immunofluorescence, 5×104 cells were cultured in Nunc Lab-Tek chamber slides (Millipore Sigma, Burlington, MA, USA) for 24 hours. The cells were washed and incubated overnight with rabbit anti-human VEGFR1 polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA). Next day, excess primary antibody was washed and goat anti-rabbit antibody conjugated to Alexa Fluor 488 (Life Technologies, Eugene, OR, USA) was used to visualize VEGFR1. Nuclei were stained with Vectashield Mounting Medium containing DAPI (Vector Laboratories, Burlingame, CA, USA).
Rabbit anti human vegfr1 polyclonal antibody
Manufactured by Santa Cruz Biotechnology
Sourced in United States
The Rabbit anti-human VEGFR1 polyclonal antibody is a research-use laboratory reagent. It is designed to detect and bind to the human VEGFR1 protein, also known as Flt-1. This antibody can be used in various immunoassay techniques to study the expression and distribution of VEGFR1 in biological samples.
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Lab products found in correlation
2 protocols using rabbit anti human vegfr1 polyclonal antibody
1
Visualizing VEGFR1 Expression in Cells
2
Visualizing VEGFR1 Expression in Cells
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