The levels of serum IgE as well as tissue IL-1β and TGF-β were measured using mouse IgE ELISA kit (cat. no. ab157718; Abcam, Cambridge, MA, USA), mouse IL-1β ELISA kit (cat. RAB0275; Millipore/Sigma, Burlington, MA, USA), and mouse TGF-β1 ELISA kit (cat. LS-F5184-1; LifeSpan BioSciences, Seattle, WA, USA), respectively. The level of IL-13, IL-4 and TNF-α in BALF and ex vivo cultured cell media were measured using mouse IL-4 Quantikine ELISA Kit (cat. no. SM4000B1; R&D Systems, Minneapolis, MN, USA), mouse IL-13 ELISA Kit (cat. no. BMS6015; Invitrogen/Thermo Fisher Scientific, Wilmington, DE, USA) and mouse TNF-α Quantikine ELISA Kit (cat. no. SMTA00B; R&D Systems), respectively.
Mouse il 13 elisa kit
Manufactured by R&D Systems
Sourced in United States
The Mouse IL-13 ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the detection and quantification of mouse interleukin-13 (IL-13) in cell culture supernatants, serum, and plasma.
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3 protocols using mouse il 13 elisa kit
1
Quantifying Inflammatory Mediators in Mice
2
Cytokine Profiling of Lung CD4+ T Cells
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Lung CD4+CD3e+ cells from the mice immunized with ascr#7 or control (solvent vehicle) were purified with a FACSAria cell sorter (Becton Dickinson and Company). Cells were cultured with OVA plus irradiated splenic antigen-presenting cells (APCs) for 96 h. The supernatants were collected, and the concentrations of IL-4, IL-5, and IFN-γ were measured by an ELISA, as described previously (37 (link)). The production of IL-13 was evaluated with a mouse IL-13 ELISA Kit (R&D Systems) according to the manufacturer’s protocol.
3
Quantifying Mouse IL-13 via ELISA
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ELISA quantification of the Mouse IL-13 ELISA Kit (M1300CB, R&D systems Inc., Minneapolis, MN, USA) was performed according to the manufacturer’s instructions.
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