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Nod scid il2rg nsg

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The NOD/SCID/IL2rg−/− (NSG) is a type of immunodeficient mouse model. It is characterized by the absence of functional T cells, B cells, and natural killer cells, as well as impaired innate immunity. This model is commonly used in various research applications, including the study of human cell engraftment and the evaluation of therapeutic interventions.

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Cd151, by BioLegend (2 mentions) C57bl 6j, by Jackson ImmunoResearch (1 mentions)

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NOD/SCID (81 citations) NOD-scid /Il2rg−/− (NSG) mice (26 citations) NOD-SCID-IL2Rg-/- (NSG) female mice (4 citations) NOD.Cg-Prkdc scid Il2rg tm1Wjl/SzJ (NSG) female mice (3 citations) Female BLT-NOD-scid IL2Rg−/− (NSG) mice (2 citations)

3 protocols using nod scid il2rg nsg

1

Engraftment and Tumor Implantation in NSG Mice

Cited 2 times
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Donated, de-identified cord blood was obtained from the University of Colorado cord blood bank (http://www.clinimmune.com/cordbloodbank/). G-CSF-mobilized patient blood was collected in accordance with the protocols approved by the Colorado Multiple Institutional Review Board (COMIRB #08-0552 and #06-0720). HSPCs were expanded as previously reported (18 (link)). NOD/SCID/IL2rg−/− (NSG; Jackson Laboratories, Bar Harbor, ME) mice were prepared as previously described (18 (link)). After eight weeks, mice were bled via the tail vein to assess HSPC engraftment. The peripheral blood was analyzed by flow cytometry, using human CD3, CD45, and/or CD151 (Biolegend, San Diego, CA) antibodies. Tumor tissue was collected and implanted on all mice as described (19 (link)).
Morton J.J., Bird G., Keysar S.B., Astling D.P., Lyons T.R., Anderson R.T., Glogowska M.J., Estes P., Eagles J.R., Le P.N., Gan G., McGettigan B., Fernandez P., Padilla-Just N., Varella-Garcia M., Song J.I., Bowles D.W., Schedin P., Tan A.C., Roop D.R., Wang X.J., Refaeli Y, & Jimeno A. (2015). XactMice: humanizing mouse bone marrow enables microenvironment reconstitution in a patient-derived xenograft model of head and neck cancer. Oncogene, 35(3), 290-300.
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2

Engraftment and Tumor Implantation in NSG Mice

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Donated, de-identified cord blood was obtained from the University of Colorado cord blood bank (http://www.clinimmune.com/cordbloodbank/). G-CSF-mobilized patient blood was collected in accordance with the protocols approved by the Colorado Multiple Institutional Review Board (COMIRB #08-0552 and #06-0720). HSPCs were expanded as previously reported (18 (link)). NOD/SCID/IL2rg−/− (NSG; Jackson Laboratories, Bar Harbor, ME) mice were prepared as previously described (18 (link)). After eight weeks, mice were bled via the tail vein to assess HSPC engraftment. The peripheral blood was analyzed by flow cytometry, using human CD3, CD45, and/or CD151 (Biolegend, San Diego, CA) antibodies. Tumor tissue was collected and implanted on all mice as described (19 (link)).
Morton J.J., Bird G., Keysar S.B., Astling D.P., Lyons T.R., Anderson R.T., Glogowska M.J., Estes P., Eagles J.R., Le P.N., Gan G., McGettigan B., Fernandez P., Padilla-Just N., Varella-Garcia M., Song J.I., Bowles D.W., Schedin P., Tan A.C., Roop D.R., Wang X.J., Refaeli Y, & Jimeno A. (2015). XactMice: humanizing mouse bone marrow enables microenvironment reconstitution in a patient-derived xenograft model of head and neck cancer. Oncogene, 35(3), 290-300.
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3

Neurological Impairment in NSG and C57BL/6J Mice

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All animal experiments were approved by the City of Hope Institutional Animal Care and Use Committee. Mice were sacrificed when they became moribund with neurologic impairments or obvious weight loss (up to 20%). NOD/SCID/IL-2rg (NSG) and C57BL/6J mice were purchased from The Jackson Laboratory. Details are included in Supplementary Materials.
Ma R., Lu T., Li Z., Teng K.Y., Mansour A.G., Yu M., Tian L., Xu B., Ma S., Zhang J., Barr T., Peng Y., Caligiuri M.A, & Yu J. (2021). An oncolytic virus expressing IL-15/IL-15Rα combined with off-the-shelf EGFR-CAR NK cells targets glioblastoma. Cancer research, 81(13), 3635-3648.
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