N hexane

All materials for culture medium and chemicals, such as n-hexane, methanol, chloroform, picric acid, sodium carbonate, and Nile red were bought from Hi-Media Laboratories (Mumbai, India). PCR primers and FAME standards were purchased from Sigma-Aldrich (St Louis, MO, USA).

Ethanol, mEthanol, chloroform, petroleum ether, ethyl acetate, diethyl ether, acetone, n-hexane, gallic acid standard, rutin standard, ascorbic acid, Folin–Ciocalteu’s phenol reagent, sodium carbonate, sodium nitrite, sodium hydroxide, aluminium chloride, ferric chloride, and potassium persulfate were obtained from Himedia Laboratories, Mumbai, India. 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,4,6-tripyridyl-s-triazine (TPTZ), 2, 2-diphenyl-1-picrylhydrazyl (DPPH) were obtained from Sigma Aldrich, Bengaluru, India. All other analytical grade chemicals and reagents were obtained from Himedia Laboratories, Mumbai, India. Distilled water was used for all experiments.

Partial purification of the bioactive metabolites of the CEL7 extract was performed using thin-layer chromatographic techniques. The fungal extract was prepared as described earlier. The dried fungal extract was resuspended in EA, maintaining a concentration of 20 mg ml⁻¹, and 10 µl of EA extract was loaded onto alumina–silica Thin Layer Chromatographic (TLC) plates (MERCK Silica Gel F254) using capillary glass tubes. Acetone (HiMedia) and n-hexane (HiMedia) in a ratio of 2:8 were used as running solvents, and the retention factor was calculated for all the bands under UV light using a Camag UV Cabinet. The bioactive compounds corresponding to each band were scratched and collected, and they were finally dissolved in 1 ml of EA, which was centrifuged (7,000 rpm for 15 min) and evaporated to dryness. The dried components were then dissolved in DMSO at 100 µg ml⁻¹ concentration, and the disk diffusion technique measured antifungal action (Wang et al., 2014 (link)).