Urinary creatinine levels were determined using the LabAssay Creatinine assay (Wako, Japan). The kit was mixed with diluted urine and reagents using the Jaffe reaction. Urinary creatinine levels were quantified by measuring the absorbance at 520 nm using a microplate reader (SUNRISE) from TECAN (Tecan Group Ltd., Männedorf, Switzerland).
Labassay creatinine assay
Manufactured by Fujifilm
Sourced in Japan
The LabAssay Creatinine assay is a diagnostic laboratory equipment used to measure the concentration of creatinine in biological samples, such as blood or urine. Creatinine is a waste product that is filtered out by the kidneys, and its measurement is an important indicator of kidney function. The LabAssay Creatinine assay provides a quantitative analysis of creatinine levels, which can be used by healthcare professionals to assess and monitor kidney health.
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Lab products found in correlation
2 protocols using labassay creatinine assay
1
Urinary Creatinine Measurement Protocol
2
Creatinine Quantification in Urine
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The creatinine concentration inside urine samples were determined by LabAssay™ Creatinine assay (Wako, Japan). Briefly, urine samples and standards were thawed, deproteinized and centrifuged. The supernatant was separated and reacted with picric acid in alkaline solution to produce tangerine condensate through Jaffe reaction. [22 ] Quantitation of total creatinine inside samples was made by measurement of absorbance by a Clariostar Monochromator Microplate Reader (BMG Labtech, Hong Kong). Concentrated urine samples which exceeded the calibration points were diluted with water with appropriate dilution factors before sample preparation. Each sample was determined at least twice with RSD less than 15%.
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