Purification using RP-HPLC was performed using a Shimadzu High-Performance Liquid Chromatograph equipped with a SPD-M20A Prominence Photo Diode Array Detector and two LC-20AP pumps running lab solutions v5.97SP1. Preparative separations were performed on a Waters XBridge BEH300 Prep C18 column (5 μm, 19 × 150 mm) at a flow rate of 10 mL/min. The solvents used were water + 0.1% formic acid (solvent A) and HPLC-grade ACN + 0.1% formic acid (solvent B).
Xbridge beh300 prep c18 column
Manufactured by Waters Corporation
The XBridge BEH300 Prep C18 column is a high-performance liquid chromatography (HPLC) column designed for preparative-scale separations. It features a bridged ethyl hybrid (BEH) particle technology and a C18 bonded stationary phase. The column is intended for use in the purification and isolation of compounds in a variety of applications.
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3 protocols using xbridge beh300 prep c18 column
1
Preparative RP-HPLC Purification Protocol
2
Compound Purification by HPLC
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Compound purification was performed using a Shimadzu High Performance Liquid Chromatograph equipped with a SPD-M20A Prominence Photo Diode Array Detector and two LC-20AP pumps. Purification used a Waters XBridge BEH300 Prep C18 column (5 µm, 19 × 150 mm) at a flow rate of 10 mL/min. The solvents used were water + 0.1% TFA (solvent A) and ACN + 0.1% TFA (solvent B).
3
LCMS-Based Compound Characterization Protocol
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Analyses were carried out on a Shimadzu High-Performance Liquid Chromatograph coupled to a Mass Spectrometer LCMS-2020 (ESI, operating both in positive and negative mode) equipped with an SPD-20A Prominence Photo Diode Array Detector and a LC-20AD solvent delivery module running lab solutions v5.97SP1. Analytical separations were performed using a Waters XBridge BEH300 Prep C18 column (10 μm, 4.6 × 250 mm). The solvents used were water + 0.1% formic acid (solvent A) and HPLC-grade ACN + 0.1% formic acid (solvent B).
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