Recombinant HIV-1 gp120 protein was obtained through the AIDS Research and Reference Reagent Program, Division of AIDS, NIAID, NIH (Germantown, MD). The experimental control of gp120 was prepared by boiling gp120 for 10 min to inactivate its protein activity while preserving its inherent endotoxin activity. Slit2N was purchased from PreproTech, Inc. (Rocky Hill, NJ). Cocaine hydrochloride (#C5776) was obtained from Sigma-Aldrich Corp. (St. Louis, MO). Double distilled water was the vehicle control used for Cocaine hydrochloride. Specific inhibitors for the dopamine-4 receptor (Sonepiprazole hydrate) and the sigma-1 receptor (Metaphit methanesulfonate salt) were purchased from Sigma-Aldrich. Anti-phospho-NFκB, anti-phospho-c-Src, anti-CCR5, anti-CXCR4, and anti-albumin antibodies were purchased from Cell Signaling Technology, Inc. (Beverly, MA). Anti-phospho-paxillin, anti-D2-40, and anti-Robo4 antibodies were purchased from Abcam Inc. (Cambridge, MA). VEGF-C was purchased from R&D Systems, Inc. (Minneapolis, MN). All other antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA).
Anti robo4
Manufactured by Abcam
Sourced in China
Anti-Robo4 is a primary antibody that targets the Robo4 protein. Robo4 is a member of the roundabout receptor family and functions as a regulator of angiogenesis. The Anti-Robo4 antibody can be used to detect and study the Robo4 protein in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti ccr5, by Cell Signaling Technology (1 mentions)
Anti cxcr4, by Cell Signaling Technology (1 mentions)
Slit2n, by Thermo Fisher Scientific (1 mentions)
Anti phospho c src, by Cell Signaling Technology (1 mentions)
Cocaine hydrochloride, by Merck Group (1 mentions)
Anti phospho nf κb, by Cell Signaling Technology (1 mentions)
Vegf c, by R&D Systems (1 mentions)
Anti slit2, by Abcam (1 mentions)
Hrp conjugated secondary antibody, by Proteintech (1 mentions)
Cell lysis buffer, by Sangon (1 mentions)
Anti β actin ab6276, by Abcam (1 mentions)
2 protocols using anti robo4
1
HIV-1 gp120 Protein Inactivation and Signaling
2
Intestinal Protein Profiling via Western Blot
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total protein was isolated from the intestine by homogenization in cell lysis buffer (Sangon Biotech Co., Ltd., Shanghai, China), and then centrifuged at 12,000 × g for 30 min at 4 ℃. The proteins (5 μg/μL) were separated by 10% SDS-PAGE in Tris-glycine-SDS buffer. Separated proteins were transferred to NC membranes which were blocked with 5% skim milk (Bright Dairy Co., Ltd, China) for one hour at room temperature and then incubated with a 1:500 dilution of the individual primary antibodies anti-Slit2 (ab134166), anti-Robo4 (ab180824), anti-VEGF (ab150375), anti-NLRP3 (ab263899), anti-ASC (ab2236), anti-Caspase1 (ab207802), anti-ZO1 (ab61357), anti-Claudin5 (ab131259), anti-Occludin (ab167161), and anti-β-actin (ab6276) (Abcam, CA, USA). The antibodies were diluted in 3% BSA in Tris-buffered saline containing 0.1% Tween 20 (TBST), and applied overnight at 4℃. Membranes were washed and incubated with HRP-conjugated secondary antibodies (Proteintech, USA). Protein bands were detected using the Western Blot Luminol Reagent (Bio-rad, USA).
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