1200 quadrupole mass spectrometer

The phytoconstituents of K. fedtschenkoi extracts were identified using a Varian CP-3800 gas chromatograph coupled to a Varian 1200 quadrupole mass spectrometer [77 (link)]. Samples were injected into a Factor Four (VF-5 ms, 30 m × 0.25 mm, 0.25 μm thickness) capillary column. Helium was used as the carrier gas at a 1 mL/min flow rate. The separation was carried out by injecting 1 μL of the sample (1%) into the column at the following gradient temperature: 60 °C for 2 min, 120 °C for 16 min, 30 °C/min up to 150 °C for 15 min, 20 °C/min up to 180 °C for 15 min, 30 °C/min up to 200 °C for 10 min, 20 °C/min up to 220 °C for 15 min, 5 °C/min up to 280 °C for 20 min, and 5 °C/min up to 300 °C for 20 min. Individual components from the extracts were identified based on comparing their retention times and fragmentation patterns to the National Institute of Standards and Technology Mass Spectral (NIST-MS) database. The total area of the peaks assessed their relative percentage.

The extracts’ chemical profiles of T. vanhouttei were established using a Varian CP-3800 gas chromatograph coupled to a Varian 1200 quadrupole mass spectrometer. The extracts were analyzed according to published protocols [55 (link)]. Briefly, 1 µL of samples prepared at 1% (w/v) chloroform were injected into a Factor Four capillary column: VF-5MS (5% phenylmethyl polysiloxane–95% polydimethylsiloxane; Agilent Technologies), 30 m × 0.25 mm, and 0.25 µm thickness. The separation of phytoconstituents was achieved by using helium as the carrier gas (1 mL/min flow rate) at the following gradient temperature: 60 °C for 2 min, 120 °C for 16 min, 160 °C for 15 min, 180 °C for 15 min, 200 °C for 10 min, 230 °C for 15 min, 290 °C for 20 min, and 300 °C for 30 min. The extracts’ components were determined according to their fragmentation patterns and retention times by consulting the National Institute of Standards and Technology Mass Spectral (NIST-MS) database. The relative percentage of phytoconstituents was registered based on the total area of the peaks.