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4 protocols using ir780 iodide

1

In Vitro Cell Viability Assay

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Zein (Meilun Biological, Dalian, China). N2'-deacetyl-N2'-(3-mercapto-1-oxopropyl)-maytansine (DM1 > 98%, Bright Gene Co., Ltd., Suzhou, China). Dimethyl sulfoxide (DMSO, Bailunsi, Tianjin, China). 2-[2-[2-Chloro-3-[(1,3-dihydro-3,3-dimethyl-1-propyl-2H-indol-2-ylidene) ethylidene]-1-cyclohexen-1-YL] ethenyl]-3,3-dimethyl-1-propylindolium iodide (IR-780 iodide, Alfa Aesar, Tianjin, China). Hoechst (Beyotime Biotechnology, Shanghai, China). Cell Counting Kit-8 (CCK-8, Dojindo, Shanghai, China). Dulbecco's modified eagle's medium (DMEM, Solarbio, Beijing, China). Fetal bovine serum (FBS, Gibco, Grand Island, NY). Trypsin-EDTA (Gibco). All materials were used without further purification.
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2

Multifunctional Nanoparticle-based Gene Delivery

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Resomer RG502H Poly (D,L-lactide-co-glycolide, acid terminated), Polyethylenimine (branched, Mw≈25000), Poly (vinyl alcohol), Ethylcarbodiimide hydrochloride were purchased from Sigma Aldrich (product of Germany). Sulfo-NHS was purchased from Thermo Fisher Scientific (USA). Lipofectamine 2000 Reagent was purchased from Invitrogen (Thermo Fisher Scientific, USA). IR780 iodide was purchased from Alfa Aesar (product of Ukraine). EndoFree Maxi Plasmid Kit was purchased from TIANGEN BIOTECH Co. Ltd (Beijing, China). PHF2-pCDNA3.1-EGFP-Amp plasmid was constructed in TINGJUNTECH (Thermo Fisher Scientific, Shanghai, China). Isopropyl alcohol was purchased from MACKLIN Biochemical Co. Ltd (Shanghai, China). Cell Counting Kit 8 (CCK8), Gel-Red, DAPI, DiI were purchased from Beyotime Technology. Doxorubicin hydrochloride was purchased from Adamas Reagent Co, Ltd. (Shanghai, China). PHF2 primary antibody was purchased from Cell Signaling Technology (USA). E-cadherin/Vimentin/ERα/CX43 primary antibody were purchased from ServiceBio (Wuhan, China).
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3

Lipid-Polymer Nanocarrier for miRNA Delivery

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Poly(lactic-co-glycolic acid)-Polyethylenimine (branched, Mw≈25000), was obtained from Ruixi (Xi’an, China). Trichloromethane, methanol, and dimethyl sulfoxide (DMSO) were provided by Aladdin. Lipofectamine 2000 Reagent was purchased from Invitrogen (Thermo Fisher Scientific, USA). IR780 iodide was purchased from Alfa Aesar (product of Ukraine). miR338-3p mimics was obtained from Ribo (Guangzhou, China). Cell Counting Kit 8 (CCK8), Gel-Red, DAPI, DiI were purchased from Beyotime Technology.
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4

Encapsulation and Characterization of Lactobacillus rhamnosus GG

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Lactobacillus rhamnosus GG (LGG) was obtained from The Korean Collection for Type Cultures (KCTC 5033; Jeongeup, South Korea). De Man, Rogosa, and Sharpe (MRS) broth and peptone were purchased from BD Biosciences (San Jose, California). Sodium alginate (molecular weight, 80,000–120,000 Da; mannuronate:guluronate ratio, 1:56), sodium chloride, sodium citrate tribasic, sodium hydroxide, bromophenol blue (BPB), vancomycin hydrochloride, and vegitone MRS agar were purchased from Sigma‐Aldrich (St. Louis, Missouri). Calcium chloride dihydrate was purchased from Kanto Chemical Co. Ltd. (Tokyo, Japan). Agar was purchased from Yakuri Pure Chemicals Co. Ltd. (Kyoto, Japan). Diammonium hydrogen phosphate, dibasic potassium phosphate, and hydrochloric acid were purchased from Junsei Chemical (Tokyo, Japan). IR780 iodide was purchased from Alfa Aesar (Ward Hill, Massachusetts). All reagents and solvents used were of the highest grade commercially available.
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