Alexa fluor 488 donkey anti rabbit igg antibody a21206

Recombinant mouse netrin-1 (1109-N1-025) was purchased from R&D systems (Minneapolis, MN, USA). Anti-netrin-1 antibodies (ab126729 and ab122903), anti-DCC antibody (ab125280), anti-UNC5H2 antibody (ab189914), anti-KIF1A antibody (ab180153), and Ms mAb to NeuN (ab104224) were purchased from Abcam (Cambridge, MA, USA). Cleaved caspase-3 (Asp175) antibody (9661) was purchase from Cell Signaling Technology (Danvers, MA, USA). Normal rabbit IgG (sc-2027) and anti-β-tubulin antibody (sc-365791) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). An ELISA kit for netrin-1 was purchased from Cloud-Clone (SEB827Ra, Houston, TX, USA). Secondary antibodies for immunofluorescence microscopy, including Alexa Fluor-488 donkey anti-rabbit IgG antibody (A21206), Alexa Fluor-555 donkey anti-mouse IgG antibody (A31570), Alexa Fluor-488 donkey anti-goat IgG antibody (A11055), and Alexa Fluor-647 donkey anti-rabbit IgG antibody (ab150075) were obtained from Invitrogen (Carlsbad, CA, USA). Secondary antibodies for western blot analysis, including anti-rabbit IgG horseradish peroxidase (HRP)-linked antibody (7074) and anti-mouse IgG HRP-linked antibody (7076) were purchased from Cell Signaling Technology.

Rabbit anti-GPR18 polyclonal antibody (SAB4501252) was provided by Sigma. Rabbit anti-albumin polyclonal antibody (ab51422), rabbit anti-eNOS polyclonal antibody (ab66127), rabbit anti-nNOS monoclonal antibody (ab76067), rabbit anti-5-LOX monoclonal antibody (ab169755), mouse anti-NeuN antibody (ab104224), mouse anti-GFAP antibody (ab10062), and mouse anti-von Willebrand factor (vWF) antibody (ab68545) were purchased from Abcam (Cambridge, MA, USA). Rabbit anti-5-LOX (phospho-Ser663) polyclonal antibody (P12527) was purchased from Biorbyt (Cambridge, CA, UK). Rabbit anti-ERK1/2 (phospho-Thr202/Tyr204) antibody (#4370) and monoclonal rabbit anti-β-tubulin antibody (#2146) were provided by Cell Signaling Technology (Danvers, MA, USA). Rabbit anti-ZO-1 polyclonal antibody (RA231621) was purchased from Thermo Fisher (Waltham, MA, USA). Goat anti-rabbit IgG HRP (sc-2004) was purchased from Santa Cruz Biotechnology (CA, USA). Alexa Fluor-488 donkey anti-rabbit IgG antibody (A21206) and Alexa Fluor-555 donkey anti-mouse IgG antibody (A31570) were purchased from Invitrogen (Carlsbad, CA, USA).

Antibodies against cleaved caspase-3 (9661), LC3 (2775), and Atg7 (2631) were purchased from Cell Signaling Technology. Sec22b antibody (14776-1-AP) was purchased from Proteintech. Ykt6 antibody (ab236583), NeuN antibody (ab104224), and GFAP antibody (ab134436) were from Abcam. CD31 antibody (AF3628) was purchased from R&D Systems. Antibodies against β-tubulin (sc-5274), normal rabbit IgG (sc-2027), and horseradish peroxidase-conjugated secondary antibodies were purchased from Santa Cruz Biotechnology. Alexa Fluor-488 donkey anti-rabbit IgG antibody (A21206), Alexa Fluor-555 donkey anti-mouse IgG antibody (A31570), and Alexa Fluor-555 donkey anti-goat IgG antibody (A21432) were purchased from Invitrogen.