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Aif 1

Manufactured by Fujifilm
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Sourced in Japan, United States

The AIF-1 is a laboratory equipment product manufactured by Fujifilm. It is designed for the analysis and processing of various types of samples. The core function of the AIF-1 is to perform automated image acquisition and analysis tasks.

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Lab products found in correlation

Bradford protein assay kit, by Bio-Rad (1 mentions) Endogenous peroxidase blocking, by Agilent Technologies (1 mentions) α sma, by Abcam (1 mentions) Dm6b fluorescent microscope, by Leica (1 mentions) Cd79a, by Novus Biologicals (1 mentions) Bcl 6, by Abcam (1 mentions) 880 laser scanning confocal microscope, by Zeiss (1 mentions)

3 protocols using aif 1

1

Colorectal Tissue Homogenization and Protein Analysis

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Rat colonic tissue was homogenized in ice-cold lysis buffer (50 mM Tris-HCl, pH 7.4, 5 mM EDTA, 1 mM EGTA, 1 mM PMSF, 5 μg/mL leupeptin, and 5 μg/mL aprotinin) by mechanical disruption. To remove connective tissue, centrifugation (500 rpm for 10 min at 4 °C) was carried out. Each supernatant was collected and stored at −80 °C until use. The amount of protein present was determined using a Bradford protein assay kit (Bio-Rad). Protein extracts were analyzed by SDS–PAGE and immunoblots were carried out as previously described [16 (link)] using primary antibodies against AIF-1 (Wako, Osaka, Japan), AKT (Cell Signaling Technology, Danvers, MA, USA), and albumin (Sigma-Aldrich, St. Louis, MO, USA) for serum specimens.
Guijarro L.G., Cano-Martínez D., Toledo-Lobo M.V., Ruiz-Llorente L., Chaparro M., Guerra I., Iborra M., Cabriada J.L., Bujanda L., Taxonera C., García-Sánchez V., Marín-Jiménez I., Barreiro-de Acosta M., Vera I., Martín-Arranz M.D., Mesonero F., Sempere L., Gomollón F., Hinojosa J., Zoullas S., Monserrat J., Menor-Salvan C., Alvarez-Mon M., Gisbert J.P., Ortega M.A, & Hernández-Breijo B. (2022). Evaluation of AIF-1 (Allograft Inflammatory Factor-1) as a Biomarker of Crohn’s Disease Severity. Biomedicines, 10(3), 727.
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2

Immunohistochemistry for IBA-1 Detection

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Heat treatment for 20 min was necessary before endogenous peroxidase blocking (DAKO, Glostrup, Denmark) for 5 min. Afterward, sections were incubated with a polyclonal antibody against ionized calcium binding adaptor molecule-1 (IBA-1, also known as allograft inflammatory factor-1 (AIF-1), 1:1000, overnight 4 °C; WAKO, USA).
Guijarro I.M., Garcés M., Andrés-Benito P., Marín B., Otero A., Barrio T., Carmona M., Ferrer I., Badiola J.J, & Monzón M. (2020). Assessment of Glial Activation Response in the Progress of Natural Scrapie after Chronic Dexamethasone Treatment. International Journal of Molecular Sciences, 21(9), 3231.
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3

Multicolor Immunofluorescence Staining Protocol

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Immunofluorescence staining was performed as previously described.16 (link) Primary antibodies from different species detected using secondary antibodies with minimal crossreactivity to other species were included for detection. Primary antibodies included CD20 (Thermo Scientific, RB-9013-P1), CD3 (Thermo Scientific, MA5-12577), CD19 (GenScript, produced custom), CD79A (Novus, NB100-64347SS), BCL6 (Abcam, ab172610), FOXP3 (Thermo Scientific, 14-5773-82), ferret IgM (Novus, NBP1-73398), ferret IgG (Novus, NBP1-73391), KRT8 (Origene, BP5007), pan-cytokeratins (Sigma-Aldrich, C2562), C4d (LSBio, LS-B3921), αSMA (Abcam, ab7817), AIF1 (Wako, 019-19741), and CD31 (Novus, NB100-2284). Images were taken using a Zeiss 880 laser scanning confocal microscope equipped with PMT and Airyscan detectors (Zeiss) and a Leica DM6B fluorescent microscope fitted with a DFC7000 T digital color camera (Leica). A more detailed Immunofluorescence procedure and a list of all antibodies is included in the supplementary materials (Table S2).
Lynch T.J., Ahlers B.A., Swatek A.M., Ievlev V., Pai A.C., Brooks L., Tang Y., Evans I.A., Meyerholz D.K., Engelhardt J.F, & Parekh K.R. (2022). Ferret Lung Transplantation Models Differential Lymphoid Aggregate Morphology Between Restrictive and Obstructive Forms of Chronic Lung Allograft Dysfunction. Transplantation, 106(10), 1974-1989.
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