After blood samples were obtained, they were left to stand for 1 h prior to centrifugation for 20 min at 3500 rpm. The serum was then harvested. Liver function was assessed by measuring three well-known hepatic indicators [alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP), and serum levels of urea nitrogen (BUN) and creatinine (CRE)] were determined to assess renal function. All these measurements were performed using an automated AU5400 biochemistry analyzer (Olympus, Japan).
Au5400 biochemistry analyzer
Manufactured by Olympus
Sourced in Japan
The Olympus AU5400 is a biochemistry analyzer designed for clinical laboratory use. It is capable of performing a wide range of diagnostic tests on various biological samples. The AU5400 utilizes advanced analytical techniques to provide accurate and reliable results, supporting healthcare professionals in making informed decisions.
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Lab products found in correlation
6 protocols using au5400 biochemistry analyzer
1
Evaluation of Liver and Renal Function
2
Biomarker Assessment in Fasting Subjects
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Blood samples of all these subjects were collected in the morning under fasting conditions, and sera were separated immediately after blood collection and stored at −80°C until use. Serum levels of tumor necrosis factor (TNF-α) and interleukin-6 (IL-6) were determined using the commercially available ELISA kits (R&D Systems, Minneapolis, MN). The assays were performed according to the manufacturer’s protocols. Serum iron status, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and bilirubin were determined using an automated Olympus AU5400 biochemistry analyzer (Olympus Corp., Tokyo, Japan). Hemoglobin levels and red blood cell count in blood were determined by ADVIA 120 hematology analyzer (Siemens, Healthcare Diagnostics Inc., Deerfield, IL, USA).
3
Biometrics and Blood Biomarkers Protocol
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Height (cm) and weight (kg) were measured in the standing position when health examination or admission. Venous blood samples were collected after overnight fasting for T2DM patients, healthy examination individuals and volunteers. Venous blood samples were collected for AMI patients within 4 hours of admission. Serum samples were prepared by centrifugation at 2000 rpm for 10 min at 4°C. Blood routine parameters were measured using the automatic hematology analyzer (Beckman-CoulterLH750, USA). The inter-assay coefficients of variation (CV%) amounts to 3.6% and 2.9% in the higher normal and lower normal range respectively. Fasting serum glucose (GLU), total cholesterol (TC), triglyceride (TG) and high-density lipoprotein cholesterol (HDL-c), low-density lipoprotein cholesterol (LDL-c), Serum aminotransferase (ALT) and aspartate aminotransferase (AST), serum urea nitrogen(BUN), uric acid concentrations were measured using the automated chemistry analyzer (Olympus AU5400 biochemistry analyzer, Japan). The inter-assay coefficients of variation (CV%) amounts to 3.9% and 2.7% in the higher normal and lower normal range respectively. Serum samples for FFAs assays were stored at -80°C until analysis. Serum FFA levels were measured with high pressure liquid chromatography (HPLC) within one hour after rehydration.
4
Biochemical Analysis of Murine Blood
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On 15 day of the treatment in Section 2.12, 5 mice from each group were selected randomly, and a 200 μL blood sample was collected from each mouse through the tail vein after fasting overnight. All the obtained blood samples were subjected to analysis on an automated AU5400 biochemistry analyzer (Olympus, Tokyo, Japan), and the concentrations of liver function indicators [aspartate aminotransferase (AST) and alanine aminotransferase (ALT)] and renal function indicators [creatinine (CRE) and urea nitrogen (BUN)] were determined. All procedures complied with the manufacturer's instructions.
5
Insulin Release from Nanoparticles under Glucose Conditions
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In order to determine the in vitro release, the different insulin-loaded NPs (5 mg) were mixed with PBS (20 mL; 0.1 M, pH=7.4) containing different glucose concentrations (0, 1, 2 and 3 mg/mL) and shaken (100r/min) at 37 o C. After a predetermined time, an aliquot of supernatant (1 mL) was
removed and fresh buffer solution was added. The amount of free insulin was monitored by UV spectrophotometry with each sample being analyzed in triplicate and the results reported as the mean ±standard deviation (n=3). to 120 mg/mL were added and incubated for 24 h. Then MTT solution in PBS buffer (20 L; 5 mg/mL) was added to each well and then removed four hours later. The samples in the wells were allowed to dry naturally for 4h, DMSO (200 uL) was added to dissolve the formed crystals and the optical density of the solution was measured at 570 nm using a microplate reader (Thermo Multiskan MK3, Thermo Scientific Company, Waltham, UK). The NIH 3T3 cells without any treatment were used as the control. using an automated Olympus AU5400 biochemistry analyzer (Olympus, Tokyo, Japan).
removed and fresh buffer solution was added. The amount of free insulin was monitored by UV spectrophotometry with each sample being analyzed in triplicate and the results reported as the mean ±standard deviation (n=3). to 120 mg/mL were added and incubated for 24 h. Then MTT solution in PBS buffer (20 L; 5 mg/mL) was added to each well and then removed four hours later. The samples in the wells were allowed to dry naturally for 4h, DMSO (200 uL) was added to dissolve the formed crystals and the optical density of the solution was measured at 570 nm using a microplate reader (Thermo Multiskan MK3, Thermo Scientific Company, Waltham, UK). The NIH 3T3 cells without any treatment were used as the control. using an automated Olympus AU5400 biochemistry analyzer (Olympus, Tokyo, Japan).
6
Evaluating p(NVCL-co-AAPBA)3 Toxicity in Mice
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A total of 24 Kunming mice (12 male and 12 female) weighing 19-23 g were randomly divided into experimental groups (low, medium and high doses) and the control group (n=6) and were given 100 mg/kg/d, 200 mg/kg/d and 400 mg/kg/d of p(NVCL-co-AAPBA)3 by intraperitoneal injection. By contrast, the control group was given 1 mL/kg/d saline solution by intraperitoneal injection. The eating, activity and death of the mice were observed and after 7 days of injections all mice were sacrificed and their livers, kidneys, spleens, hearts and lungs were collected for the HE staining. Transaminase (ALT) were analyzed using an automated Olympus AU5400 biochemistry analyzer (Olympus, Tokyo, Japan).
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