Sodium hydroxide naoh bioxtra

High purity reference standards of RIF (purity 98%), INH (purity 98%), and PZA (purity 98%) were obtained from Toronto Research Chemicals (Toronto, Canada). RIF (Rimactane capsule 150 mg, Sandoz, SA), INH (Winthrop-Isoniazid 300 mg, Sanofi-Aventis, SA), PZA (Macleods Pyrazinamide 500 mg, Macleods Pharmaceuticals, SA), and FDC (Rifafour e-275: RIF, INH, PZA, and EMB 150/75/400/275 mg, Sanofi-Aventis, SA), and Ryles tubes FG14 (Suprahealthcare manufacturing, Cape Town, SA) were donated by Tygerberg Hospital (Cape Town, SA). Ascorbic acid (AA), pancreas powder (lipase), pepsin, sodium chloride (NaCl) bioxtra, sodium hydroxide (NaOH) bioxtra, and potassium phosphate (KH₂PO₄) monobasic were purchased from Sigma-Aldrich (Missouri, USA). Ammonium formate (AF), acetic acid (AC), and formic acid (FA) were obtained from Fisher Chemical (Madrid, Spain). Ammonium acetate (AMAC) and hydrochloric acid (HCl) were purchased from Labchem (Johannesburg, SA). LC-MS grade acetonitrile (ACN) and methanol (MeOH) were obtained from Romil Pure Chemistry (Johannesburg, SA). A Milli-Q water purification system coupled with a Synergy UV system (Merck) was used to prepare ultrapure water. An ultrasonic bath (United Scientific) was used for the sonication of samples and mobile phases. Additionally, an Eins-Sci E-VM-A analog vortex mixer was employed to mix samples.

Phosphate buffered saline (PBS) and Penicillin‐streptomycin liquid (PSL, 100X, reagent grade, with 10 000 units penicillin and 10 000 units streptomycin) were acquired from Zhejiang Jinuo Biomedical Technology Co., Ltd. Lithium phenyl‐2,4,6‐trimethylbenzoylphosphinate (LAP) was obtained from Huaxia Siyin Biotechnology Co., Ltd., Shanghai; Gelatin (G108395 Gelatin, used in microbiology, gel strength ≈ 250 g Bloom) was bought from Aladdin; Liquid metal (Ga₂₅In₇₅) was acquired from Fanyada Electronic Technology Co., Ltd.; Ammonium sulfate ((NH₄)₂SO₄, AR, 99%) was purchased from Macklin; Electronic fluoride (ETF, MX‐056) was acquired from Suzhou Peng Rui Nano Technology Co., Ltd.; Photoreactive resin HTL (Yellow‐20) was obtained from Chongqing Boston Micro Fabrication (BMF) Technology Co., Ltd.; Polydimethylsiloxane (PDMS, Dow Corning) was from Dow Corning Ethanol (95%) was acquired from Shanghai Lanqing Industrial Co., Ltd. Type‐B gelatin, methacrylic anhydride (MA, ≥94%), sodium hydroxide (NaOH, bioXtra, ≥98%), calcein‐AM (bioreagent, ≥95.0%), and propidium iodide (PI, ≥94%) were purchased from Sigma Aldrich; fetal bovine serum (FBS, Biological Industries, 04‐001‐1A) was acquired from Hyclone, Hong Kong. Deionized (DI) water was prepared by using a laboratory water purification system.