Twenty-five mL of raw milk from bulk was diluted in 225 mL buffered peptone saline water (SRL Pvt. Ltd., Mumbai, India) and serial dilution of 10−1, 10−2, 10−3, and 10−4 was applied to quantify the coliform bacteria to assess the hygienic quality of the raw milk. The diluted milk samples (1 mL/dilution) were dispensed on the 3 M™ Petri films (3 M, USA) plates and incubated for 24–48 h at 37 °C. After 24 h of incubation, the colonies grown on the films were counted by dividing the films into four quarters to facilitate the counting. The colonies in one quarter were counted and multiplied by four and by dilution to get the total colonies per dilution. Colonies greater than 100 in one quarter were rejected as too many to count and the average load of countable dilutions was taken and converted to log1027 (link).
3 m petrifilms
Manufactured by 3M
Sourced in United States
3M™ PetriFilms™ are a compact, ready-to-use system for the enumeration of microorganisms in food and other samples. The films contain culture media and indicators that allow for the growth and visualization of target microorganisms.
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Lab products found in correlation
4 protocols using 3 m petrifilms
1
Quantifying Coliform in Raw Milk
2
Quantifying MSSA Biofilm Formation
3
Quantifying MSSA Biofilm Formation
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Two strains of methicillin-sensitive Staphylococcus aureus (MSSA) were used. ATCC®25923™ was used for all in vitro experiments whereas, to allow easy strain identification, the superfolder GFP-containing strain AH1710 [44 (link)] was used for experiments in rabbit cadavers. Overnight MSSA cultures were subcultured, 2 hr and diluted in trypticase soy broth (TSB) or human synovial fluid (SynF, “discarded” from joint aspirations (approved by TJU IRB)) to ~105 colony forming units (CFU)/ml using a 0.5 McFarland standard (turbidity standard where A600 of 0.10 yields ~1 × 108 CFU/ml). Inoculated surfaces were incubated under static conditions to facilitate biofilm formation. Upon harvesting, surfaces were gently washed 3× with PBS to remove nonadherent bacteria and either (i) fixed and visualized with scanning electron microscopy (SEM) or (ii) adherent bacteria suspended by 15 min sonication in 0.3% Tween 20 in PBS, followed by serial dilution, plating on 3 M™ PetriFilms™ (3 M Corp, St. Paul, MN), and digital recording. Dilutions with 20–500 CFU/film were counted and numbers expressed as CFU/ml.
4
Bacterial Enumeration in Pork Samples
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We performed microbial analysis with the aid of 3 M Petrifilms (3M Health Care, USA). Pork samples (25 g) were blended with 225 mL distilled water, and serially diluted. In total, 1 mL amounts of the dilutions were spread on Petrifilm Aerobic Count Plates (3M Health Care) to determine total bacterial counts (TBCs). The plates were incubated at 37±1℃ for 24-48 h. Escherichia coli was incubated on Petrifilm E. coli/Coliform Count Plates (3M Health Care) for 24-36 h under identical conditions. Bacterial colonies were counted and expressed as log10 colony-forming units (CFU)/g of meat. Counts were obtained on days 0, 1, 3, 7, 10, and 14.
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